Detection of marine neurotoxins in food safety testing using a multielectrode array

J. Nicolas, P.J.M. Hendriksen, R.G.D.M. van Kleef, A. Groot, T.F.H. Bovee, I.M.C.M. Rietjens, H.S. Westerink

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Scope At the European level, detection of marine neurotoxins in seafood is still based on ethically debated and expensive in vivo rodent bioassays. The development of alternative methodologies for the detection of marine neurotoxins is therefore of utmost importance. We therefore investigated whether and to what extent a multielectrode array (MEA) approach can be used as an in vitro alternative for screening of marine neurotoxins potentially present in seafood. Methods This MEA approach utilizes rat cortical neurons comprising a wide range of ion channels/pumps and neurotransmitter receptors targeted by marine neurotoxins. We tested the effects of neurotoxic model compounds, pure marine neurotoxins, and extracts from contaminated seafood on neuronal activity of rat cortical neurons cultured on commercial 48-well plates to increase throughput. Conclusion We demonstrate that the MEA approach has a sensitivity of 88% (7/9 model compounds, 6/6 pure marine neurotoxins, and 2/2 marine neurotoxins present in seafood extracts were correctly identified) and a good reproducibility compared to existing in vitro alternatives. We therefore conclude that this MEA-based approach could be a valuable tool for future food safety testing.
LanguageEnglish
Pages2369-2378
JournalMolecular Nutrition & Food Research
Volume58
Issue number12
DOIs
Publication statusPublished - 2014

Fingerprint

safety testing
neurotoxins
Food Safety
Neurotoxins
food safety
Seafood
seafoods
neurons
Ion Pumps
Neurons
Neurotransmitter Receptor
rats
extracts
ion channels
Ion Channels
Biological Assay
pumps
reproducibility
Rodentia
rodents

Keywords

  • microelectrode arrays
  • neuronal networks
  • sodium-channels
  • state
  • palytoxin
  • ouabain
  • toxins
  • mouse
  • cells
  • acts

Cite this

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abstract = "Scope At the European level, detection of marine neurotoxins in seafood is still based on ethically debated and expensive in vivo rodent bioassays. The development of alternative methodologies for the detection of marine neurotoxins is therefore of utmost importance. We therefore investigated whether and to what extent a multielectrode array (MEA) approach can be used as an in vitro alternative for screening of marine neurotoxins potentially present in seafood. Methods This MEA approach utilizes rat cortical neurons comprising a wide range of ion channels/pumps and neurotransmitter receptors targeted by marine neurotoxins. We tested the effects of neurotoxic model compounds, pure marine neurotoxins, and extracts from contaminated seafood on neuronal activity of rat cortical neurons cultured on commercial 48-well plates to increase throughput. Conclusion We demonstrate that the MEA approach has a sensitivity of 88{\%} (7/9 model compounds, 6/6 pure marine neurotoxins, and 2/2 marine neurotoxins present in seafood extracts were correctly identified) and a good reproducibility compared to existing in vitro alternatives. We therefore conclude that this MEA-based approach could be a valuable tool for future food safety testing.",
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Detection of marine neurotoxins in food safety testing using a multielectrode array. / Nicolas, J.; Hendriksen, P.J.M.; van Kleef, R.G.D.M.; Groot, A.; Bovee, T.F.H.; Rietjens, I.M.C.M.; Westerink, H.S.

In: Molecular Nutrition & Food Research, Vol. 58, No. 12, 2014, p. 2369-2378.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Detection of marine neurotoxins in food safety testing using a multielectrode array

AU - Nicolas, J.

AU - Hendriksen, P.J.M.

AU - van Kleef, R.G.D.M.

AU - Groot, A.

AU - Bovee, T.F.H.

AU - Rietjens, I.M.C.M.

AU - Westerink, H.S.

PY - 2014

Y1 - 2014

N2 - Scope At the European level, detection of marine neurotoxins in seafood is still based on ethically debated and expensive in vivo rodent bioassays. The development of alternative methodologies for the detection of marine neurotoxins is therefore of utmost importance. We therefore investigated whether and to what extent a multielectrode array (MEA) approach can be used as an in vitro alternative for screening of marine neurotoxins potentially present in seafood. Methods This MEA approach utilizes rat cortical neurons comprising a wide range of ion channels/pumps and neurotransmitter receptors targeted by marine neurotoxins. We tested the effects of neurotoxic model compounds, pure marine neurotoxins, and extracts from contaminated seafood on neuronal activity of rat cortical neurons cultured on commercial 48-well plates to increase throughput. Conclusion We demonstrate that the MEA approach has a sensitivity of 88% (7/9 model compounds, 6/6 pure marine neurotoxins, and 2/2 marine neurotoxins present in seafood extracts were correctly identified) and a good reproducibility compared to existing in vitro alternatives. We therefore conclude that this MEA-based approach could be a valuable tool for future food safety testing.

AB - Scope At the European level, detection of marine neurotoxins in seafood is still based on ethically debated and expensive in vivo rodent bioassays. The development of alternative methodologies for the detection of marine neurotoxins is therefore of utmost importance. We therefore investigated whether and to what extent a multielectrode array (MEA) approach can be used as an in vitro alternative for screening of marine neurotoxins potentially present in seafood. Methods This MEA approach utilizes rat cortical neurons comprising a wide range of ion channels/pumps and neurotransmitter receptors targeted by marine neurotoxins. We tested the effects of neurotoxic model compounds, pure marine neurotoxins, and extracts from contaminated seafood on neuronal activity of rat cortical neurons cultured on commercial 48-well plates to increase throughput. Conclusion We demonstrate that the MEA approach has a sensitivity of 88% (7/9 model compounds, 6/6 pure marine neurotoxins, and 2/2 marine neurotoxins present in seafood extracts were correctly identified) and a good reproducibility compared to existing in vitro alternatives. We therefore conclude that this MEA-based approach could be a valuable tool for future food safety testing.

KW - microelectrode arrays

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