Detection of intact megadalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry

W.J.H. van Berkel; R.H.H. van den Heuvel; C. Versluis; A. Heck

doi:10.1110/ps.9.3.435

Detection of intact megadalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry

W.J.H. van Berkel, R.H.H. van den Heuvel, C. Versluis, A. Heck

Research output: Contribution to journal › Article › Academic › peer-review

118 Citations (Scopus)

Abstract

Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2&Eth;The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.

Original language	English
Pages (from-to)	435-439
Journal	Protein Science
Volume	9
DOIs	https://doi.org/10.1110/ps.9.3.435
Publication status	Published - 2000

Keywords

Electrospray ionization mass spectrometry
Noncovalent interactions
Protein assemblies
Vanillyl-alcohol oxidase

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10.1110/ps.9.3.435

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title = "Detection of intact megadalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry",

abstract = "Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2&Eth;The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.",

keywords = "Electrospray ionization mass spectrometry, Noncovalent interactions, Protein assemblies, Vanillyl-alcohol oxidase",

author = "{van Berkel}, W.J.H. and {van den Heuvel}, R.H.H. and C. Versluis and A. Heck",

year = "2000",

doi = "10.1110/ps.9.3.435",

language = "English",

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pages = "435--439",

journal = "Protein Science",

issn = "0961-8368",

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AU - van Berkel, W.J.H.

AU - van den Heuvel, R.H.H.

AU - Versluis, C.

AU - Heck, A.

PY - 2000

Y1 - 2000

N2 - Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2&Eth;The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.

AB - Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2&Eth;The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.

KW - Electrospray ionization mass spectrometry

KW - Noncovalent interactions

KW - Protein assemblies

KW - Vanillyl-alcohol oxidase

U2 - 10.1110/ps.9.3.435

DO - 10.1110/ps.9.3.435

M3 - Article

SN - 0961-8368

VL - 9

SP - 435

EP - 439

JO - Protein Science

JF - Protein Science

ER -

Detection of intact megadalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry

Abstract

Keywords

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