Detection of Flowing Fluorescent Particles in a Microcapillary Using Fluorescence Correlation Spectroscopy

B.H. Kunst; A. Schots; A.J.W.G. Visser

doi:10.1021/ac0256742

Detection of Flowing Fluorescent Particles in a Microcapillary Using Fluorescence Correlation Spectroscopy

B.H. Kunst, A. Schots, A.J.W.G. Visser

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

39 Citations (Scopus)

Abstract

Capillary flow experiments are described with fluorescent molecules, bacteria, and microspheres using fluorescence correlation spectroscopy as an analytical tool. The flow velocity in the microcapillary is determined by fitting autocorrelation traces with a model containing parameters related to diffusion and flow. The flow profile of pressure-driven flow inside a microcapillary is determined by using the fluorescence fluctuations of a small dye molecule. It was found that bacteria and microspheres are retarded in their flow by optical forces produced by the laser beam.

Original language	English
Pages (from-to)	5350-5357
Journal	Analytical Chemistry
Volume	74
Issue number	20
DOIs	https://doi.org/10.1021/ac0256742
Publication status	Published - 2002

Access to Document

10.1021/ac0256742

https://edepot.wur.nl/147038Licence: Taverne

Cite this

@article{6a9049e0d8af4ef08db2aa388b2b2a05,

title = "Detection of Flowing Fluorescent Particles in a Microcapillary Using Fluorescence Correlation Spectroscopy",

abstract = "Capillary flow experiments are described with fluorescent molecules, bacteria, and microspheres using fluorescence correlation spectroscopy as an analytical tool. The flow velocity in the microcapillary is determined by fitting autocorrelation traces with a model containing parameters related to diffusion and flow. The flow profile of pressure-driven flow inside a microcapillary is determined by using the fluorescence fluctuations of a small dye molecule. It was found that bacteria and microspheres are retarded in their flow by optical forces produced by the laser beam.",

author = "B.H. Kunst and A. Schots and A.J.W.G. Visser",

year = "2002",

doi = "10.1021/ac0256742",

language = "English",

volume = "74",

pages = "5350--5357",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "20",

}

TY - JOUR

T1 - Detection of Flowing Fluorescent Particles in a Microcapillary Using Fluorescence Correlation Spectroscopy

AU - Kunst, B.H.

AU - Schots, A.

AU - Visser, A.J.W.G.

PY - 2002

Y1 - 2002

N2 - Capillary flow experiments are described with fluorescent molecules, bacteria, and microspheres using fluorescence correlation spectroscopy as an analytical tool. The flow velocity in the microcapillary is determined by fitting autocorrelation traces with a model containing parameters related to diffusion and flow. The flow profile of pressure-driven flow inside a microcapillary is determined by using the fluorescence fluctuations of a small dye molecule. It was found that bacteria and microspheres are retarded in their flow by optical forces produced by the laser beam.

AB - Capillary flow experiments are described with fluorescent molecules, bacteria, and microspheres using fluorescence correlation spectroscopy as an analytical tool. The flow velocity in the microcapillary is determined by fitting autocorrelation traces with a model containing parameters related to diffusion and flow. The flow profile of pressure-driven flow inside a microcapillary is determined by using the fluorescence fluctuations of a small dye molecule. It was found that bacteria and microspheres are retarded in their flow by optical forces produced by the laser beam.

U2 - 10.1021/ac0256742

DO - 10.1021/ac0256742

M3 - Article

SN - 0003-2700

VL - 74

SP - 5350

EP - 5357

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 20

ER -

Detection of Flowing Fluorescent Particles in a Microcapillary Using Fluorescence Correlation Spectroscopy

Abstract

Access to Document

Fingerprint

Cite this