Abstract
Rabbit antisera against the nucleocapsid protein (NP) have been commonly
used for detection of tospoviruses and classification into serogroups or serotypes.
Mouse monoclonal antibodies (MAbs) with high specificity to the NPs have also been
widely used to identify tospovirus species. Recently, a serogroup-specific MAb against
the NSs protein of Watermelon silver mottle virus (WSMoV) was produced by our
laboratory to react with five members of WSMoV serogroup, i.e., WSMoV, Capsicum
chlorosis virus (CaCV), Calla lily chlorotic spot virus (CCSV), Peanut bud necrosis virus
(PBNV) and Watermelon bud necrosis virus (WBNV). The epitope recognized by the
NSs MAb was determined and the comparison with the reported sequences of
tospoviral NSs proteins revealed that the epitope is highly conserved at the N-terminal
region of NSs proteins among members of WSMoV and Iris yellow spot virus (IYSV)
serogroups, and Melon yellow spot virus (MYSV) serotype. When the NSs MAb was
further used to react with the crude antigens of MYSV serotype, IYSV and Tomato
yellow ring virus (TYRV) of IYSV serogroup, strong serological reactions, both in
ELISA and western blotting, were observed. Thus, our results indicated that the NSs
MAb is a useful and convenient tool for detection of the eight tospovirus species. It is
also suggested that these eight Asian-type tospoviruses, i.e., WSMoV, CaCV, CCSV,
PBNV, WBNV, MYSV, IYSV and TYRV, may share a common evolutionary ancestor
Original language | English |
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Title of host publication | Proceedings of the XII International Symposium on Virus Diseases of Ornamental Plants, Haarlem, The Netherlands, 20-24 April 2008 |
Pages | 61-66 |
Volume | 901 |
DOIs | |
Publication status | Published - 2008 |
Event | XII International Symposium on Virus diseases of ornamental plants - Haarlem, Netherlands Duration: 20 Apr 2008 → 24 Apr 2008 |
Conference/symposium
Conference/symposium | XII International Symposium on Virus diseases of ornamental plants |
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Country/Territory | Netherlands |
City | Haarlem |
Period | 20/04/08 → 24/04/08 |
Keywords
- Monoclonal antibodies
- NSs protein
- Serological detection
- Tospovirus