Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor

M.E. Thomas, A. Bouma, E. van Eerden, W.J.M. Landman, F. van Knapen, J.A. Stegeman, A.A. Bergwerff

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

A surface plasmon resonance (SPR) biosensor assay was developed on the basis of a lipopolysaccharide antigen of Salmonella enterica serovar enteritidis (S. enterica serovar enteritidis) to detect egg yolk antibodies against S. enterica serovar enteritidis. This biosensor assay was compared to two commercial ELISA kits based on LPS antigen and flagellar antigen. A number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with S. enterica serovar enteritidis and 90 egg yolk and combined egg white and yolk samples from uninfected chickens were analyzed. Receiver operating characteristic analysis of the data calculated a diagnostic sensitivity of 82% and a diagnostic specificity of 100%. The within-day coefficient of variation of a positive internal-control egg yolk was 1%. The SPR biosensor assay was able to detect antibodies in a significantly higher percentage of known positive samples than the commercial ELISA's. The anticipated use of the SPR biosensor assay is to determine the S. enterica serovar enteritidis serostatus of non-vaccinated layer hens
LanguageEnglish
Pages68-74
Number of pages7
JournalJournal of Immunological Methods
Volume315
Issue number1-2
DOIs
Publication statusPublished - 2006

Fingerprint

Salmonella enteritidis
Egg Yolk
Surface Plasmon Resonance
Salmonella Infections
Biosensing Techniques
Antibodies
Egg White
Antigens
Chickens
Enzyme-Linked Immunosorbent Assay
ROC Curve
Lipopolysaccharides

Keywords

  • laying hens
  • typhimurium
  • chickens
  • elisa
  • assay
  • immunosensor
  • netherlands
  • antigens
  • serum

Cite this

Thomas, M.E. ; Bouma, A. ; van Eerden, E. ; Landman, W.J.M. ; van Knapen, F. ; Stegeman, J.A. ; Bergwerff, A.A. / Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor. In: Journal of Immunological Methods. 2006 ; Vol. 315, No. 1-2. pp. 68-74.
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title = "Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor",
abstract = "A surface plasmon resonance (SPR) biosensor assay was developed on the basis of a lipopolysaccharide antigen of Salmonella enterica serovar enteritidis (S. enterica serovar enteritidis) to detect egg yolk antibodies against S. enterica serovar enteritidis. This biosensor assay was compared to two commercial ELISA kits based on LPS antigen and flagellar antigen. A number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with S. enterica serovar enteritidis and 90 egg yolk and combined egg white and yolk samples from uninfected chickens were analyzed. Receiver operating characteristic analysis of the data calculated a diagnostic sensitivity of 82{\%} and a diagnostic specificity of 100{\%}. The within-day coefficient of variation of a positive internal-control egg yolk was 1{\%}. The SPR biosensor assay was able to detect antibodies in a significantly higher percentage of known positive samples than the commercial ELISA's. The anticipated use of the SPR biosensor assay is to determine the S. enterica serovar enteritidis serostatus of non-vaccinated layer hens",
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Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor. / Thomas, M.E.; Bouma, A.; van Eerden, E.; Landman, W.J.M.; van Knapen, F.; Stegeman, J.A.; Bergwerff, A.A.

In: Journal of Immunological Methods, Vol. 315, No. 1-2, 2006, p. 68-74.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor

AU - Thomas, M.E.

AU - Bouma, A.

AU - van Eerden, E.

AU - Landman, W.J.M.

AU - van Knapen, F.

AU - Stegeman, J.A.

AU - Bergwerff, A.A.

PY - 2006

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N2 - A surface plasmon resonance (SPR) biosensor assay was developed on the basis of a lipopolysaccharide antigen of Salmonella enterica serovar enteritidis (S. enterica serovar enteritidis) to detect egg yolk antibodies against S. enterica serovar enteritidis. This biosensor assay was compared to two commercial ELISA kits based on LPS antigen and flagellar antigen. A number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with S. enterica serovar enteritidis and 90 egg yolk and combined egg white and yolk samples from uninfected chickens were analyzed. Receiver operating characteristic analysis of the data calculated a diagnostic sensitivity of 82% and a diagnostic specificity of 100%. The within-day coefficient of variation of a positive internal-control egg yolk was 1%. The SPR biosensor assay was able to detect antibodies in a significantly higher percentage of known positive samples than the commercial ELISA's. The anticipated use of the SPR biosensor assay is to determine the S. enterica serovar enteritidis serostatus of non-vaccinated layer hens

AB - A surface plasmon resonance (SPR) biosensor assay was developed on the basis of a lipopolysaccharide antigen of Salmonella enterica serovar enteritidis (S. enterica serovar enteritidis) to detect egg yolk antibodies against S. enterica serovar enteritidis. This biosensor assay was compared to two commercial ELISA kits based on LPS antigen and flagellar antigen. A number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with S. enterica serovar enteritidis and 90 egg yolk and combined egg white and yolk samples from uninfected chickens were analyzed. Receiver operating characteristic analysis of the data calculated a diagnostic sensitivity of 82% and a diagnostic specificity of 100%. The within-day coefficient of variation of a positive internal-control egg yolk was 1%. The SPR biosensor assay was able to detect antibodies in a significantly higher percentage of known positive samples than the commercial ELISA's. The anticipated use of the SPR biosensor assay is to determine the S. enterica serovar enteritidis serostatus of non-vaccinated layer hens

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KW - assay

KW - immunosensor

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