Detection of Campylobacter Species in Bivalve Mollusks

A.H.A.M. van Hoek, W.M. van Overbeek, E.M. Bouw, P. de Graag, W.F. Jacobs-Reitsma, H.J.M. Aarts

    Research output: Chapter in Book/Report/Conference proceedingAbstract

    Abstract

    Illnesses due to bivalve mollusks consumption are most often associated with the presence of viruses; however, pathogenic bacteria like Campylobacter also have to be considered as causative agents. The overall objective of this study was to develop a procedure for the rapid detection and identification of Campylobacter species in bivalve mollusks. Research was mainly focused on oysters since these are very often consumed raw, and as such they potentially present a considerable risk to the consumers. Initially, the ISO 10272:2006 microbiological method for the detection and quantification of Campylobacter was executed and optimized. Furthermore, published real-time PCR tests (1, 2) were implemented to determine the presence of Campylobacter spp. and to identify Campylobacter coli, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. In a pilot experiment six Campylobacterpositive Illnesses due to bivalve mollusks consumption are most often associated with the presence of viruses; however, pathogenic bacteria like Campylobacter also have to be considered as causative agents. The overall objective of this study was to develop a procedure for the rapid detection and identification of Campylobacter species in bivalve mollusks. Research was mainly focused on oysters since these are very often consumed raw, and as such they potentially present a considerable risk to the consumers. Initially, the ISO 10272:2006 microbiological method for the detection and quantification of Campylobacter was executed and optimized. Furthermore, published real-time PCR tests (1, 2) were implemented to determine the presence of Campylobacter spp. and to identify Campylobacter coli, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. In a pilot experiment six Campylobacterpositive
    Original languageEnglish
    Title of host publicationPoster Presentations - 14th International Workshop on Campylobacter, Helicobacter and Related Organisms Rotterdam, The Netherlands, 2–5 September 2007
    EditorsM. Torrence
    Pages75-75
    Publication statusPublished - 2007
    Event14th international Workshop on Campylobacter, Helicobacter and Related organisms -
    Duration: 2 Sep 20075 Sep 2007

    Publication series

    NameZoonoses and Public Health
    PublisherWiley-Blackwell Verlag
    NumberS1
    Volume54
    ISSN (Print)1863-1959
    ISSN (Electronic)1863-2378

    Workshop

    Workshop14th international Workshop on Campylobacter, Helicobacter and Related organisms
    Period2/09/075/09/07

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