Detection of bovine meat and bone meal in animal feed at a level of 0.1%

H.J.M. Aarts, E.M. Bouw, J.B. Buntjer, J.A. Lenstra, L.W.D. van Raamsdonk

    Research output: Contribution to journalArticleAcademicpeer-review

    13 Citations (Scopus)

    Abstract

    For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR® Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected
    Original languageEnglish
    Pages (from-to)1443-1446
    JournalJournal of AOAC International
    Volume89
    Issue number6
    Publication statusPublished - 2006

    Keywords

    • species-specific dna
    • evolution

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