Detection of bovine meat and bone meal in animal feed at a level of 0.1%

H.J.M. Aarts; E.M. Bouw; J.B. Buntjer; J.A. Lenstra; L.W.D. van Raamsdonk

doi:10.1093/jaoac/89.6.1443

Detection of bovine meat and bone meal in animal feed at a level of 0.1%

H.J.M. Aarts, E.M. Bouw, J.B. Buntjer, J.A. Lenstra, L.W.D. van Raamsdonk

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR® Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected

Original language	English
Pages (from-to)	1443-1446
Journal	Journal of AOAC International
Volume	89
Issue number	6
DOIs	https://doi.org/10.1093/jaoac/89.6.1443
Publication status	Published - 2006

Keywords

species-specific dna
evolution

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abstract = "For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR{\textregistered} Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected",

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AU - Lenstra, J.A.

AU - van Raamsdonk, L.W.D.

PY - 2006

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AB - For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR® Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected

KW - species-specific dna

KW - evolution

U2 - 10.1093/jaoac/89.6.1443

DO - 10.1093/jaoac/89.6.1443

M3 - Article

SN - 1060-3271

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SP - 1443

EP - 1446

JO - Journal of AOAC International

JF - Journal of AOAC International

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ER -

Detection of bovine meat and bone meal in animal feed at a level of 0.1%

Abstract

Keywords

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