Detection of antibody-forming cells directed against Newcastle disease virus and their immunoglobulin class by double immunoenzyme histochemistry.

S.O. Al-Garib, E. Gruys, A.L.J. Gielkens, G. Koch

    Research output: Contribution to journalArticleAcademicpeer-review

    4 Citations (Scopus)

    Abstract

    Antibody-forming cells (AFCs) against Newcastle disease virus (NDV) and their immunoglobulin (Ig) class were demonstrated by a double immuno-enzyme histochemical technique. The AFCs were stained and quantified in spleen sections of chickens euthanatized at day 7 postexposure to the Roakin strain of NDV. The sections were incubated with NDV to determine the specificity of the AFCs. Bound virus was subsequently visualized with a primary monoclonal antibody (MAb), a secondary horseradish peroxidase¿conjugated MAb, and 3-amino-9-ethylcarbazole as substrate. IgM and IgA were stained with MAbs and an alkaline phosphatase (AP)-conjugated secondary antibody. IgG class antibodies were demonstrated with an AP-conjugated rabbit serum. The final substrate for the three Igs was naphthol AS-MX-phosphate and fast blue BB. About 64¿159/mm2 AFCs against NDV were detected. Of these virus-binding cells, about 55¿roduced IgM, 37¿roduced IgG, and the remainding 8¿roduced IgA.
    Original languageEnglish
    Pages (from-to)453-457
    JournalAvian Diseases
    Volume47
    Issue number2
    DOIs
    Publication statusPublished - 2003

    Keywords

    • secreting cells
    • enumeration
    • assay

    Fingerprint Dive into the research topics of 'Detection of antibody-forming cells directed against Newcastle disease virus and their immunoglobulin class by double immunoenzyme histochemistry.'. Together they form a unique fingerprint.

  • Cite this