Antibody-forming cells (AFCs) against Newcastle disease virus (NDV) and their immunoglobulin (Ig) class were demonstrated by a double immuno-enzyme histochemical technique. The AFCs were stained and quantified in spleen sections of chickens euthanatized at day 7 postexposure to the Roakin strain of NDV. The sections were incubated with NDV to determine the specificity of the AFCs. Bound virus was subsequently visualized with a primary monoclonal antibody (MAb), a secondary horseradish peroxidase¿conjugated MAb, and 3-amino-9-ethylcarbazole as substrate. IgM and IgA were stained with MAbs and an alkaline phosphatase (AP)-conjugated secondary antibody. IgG class antibodies were demonstrated with an AP-conjugated rabbit serum. The final substrate for the three Igs was naphthol AS-MX-phosphate and fast blue BB. About 64¿159/mm2 AFCs against NDV were detected. Of these virus-binding cells, about 55¿roduced IgM, 37¿roduced IgG, and the remainding 8¿roduced IgA.
|Publication status||Published - 2003|
- secreting cells
Al-Garib, S. O., Gruys, E., Gielkens, A. L. J., & Koch, G. (2003). Detection of antibody-forming cells directed against Newcastle disease virus and their immunoglobulin class by double immunoenzyme histochemistry. Avian Diseases, 47(2), 453-457. https://doi.org/10.1637/0005-2086(2003)047%5B0453:DOACDA%5D2.0.CO;2