Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene

T.J. Tobias, A. Bouma, D. Klinkenberg, A.J.J.M. Daemen, J.A. Stegeman, J.A. Wagenaar, B. Duim

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Abstract

A real-time quantitative PCR (qPCR) for detection of the apxIVA gene of Actinobacillus pleuropneumoniae was validated using pure cultures of A. pleuropneumoniae and tonsillar and nasal swabs from experimentally inoculated Caesarean-derived/colostrum-deprived piglets and naturally infected conventional pigs. The analytical sensitivity was 5 colony forming units/reaction. In comparison with selective bacterial examination using tonsillar samples from inoculated animals, the diagnostic sensitivity of the qPCR was 0.98 and the diagnostic specificity was 1.0. The qPCR showed consistent results in repeatedly sampled conventional pigs. Tonsillar brush samples and apxIVA qPCR analysis may be useful for further epidemiological studies and monitoring for A. pleuropneumoniae.
Original languageEnglish
Pages (from-to)557-560
JournalThe Veterinary Journal
Volume193
Issue number2
DOIs
Publication statusPublished - 2012

Keywords

  • multiplex pcr
  • identification
  • assay
  • transmission
  • serotype-2

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    Tobias, T. J., Bouma, A., Klinkenberg, D., Daemen, A. J. J. M., Stegeman, J. A., Wagenaar, J. A., & Duim, B. (2012). Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene. The Veterinary Journal, 193(2), 557-560. https://doi.org/10.1016/j.tvjl.2012.02.004