Detection and tentative grouping of Strawberry crinkle virus isolates

M.M. Klerks, J.L. Lindner, D. Vasková, J. Spak, J.R. Thompson, W. Jelkmann, C.D. Schoen

    Research output: Contribution to journalArticleAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    A partial sequence of the putative polymerase (L) protein of Strawberry crinkle virus (SCV), genus Cytorhabdovirus, is described. The virus protein was found to be distantly related to the L protein of the rhabdoviruses Northern cereal mosaic virus, Rice yellow stunt virus and Sonchus yellow net virus. Moreover, a tentative grouping of SCV isolates is described, based on phylogenetic analysis of a region enclosing the GDN-motif within the RNA-dependent RNA polymerase gene. A sequence homology of 98% was found for each tentative group, and heterogeneity of at least 11% was observed between both groups. The tentative grouping did not appear to be related to symptomatology or geographical origin of the isolates. Nevertheless, the use of several reverse-transcription (RT)-PCR primer sets, all directed to different regions within the SCV genome, confirmed the presumptive classification into two groups, namely group I (isolates 1554, KG and Post), and group II (isolates 1553, Hb-A1, 37-1 and 37-2). Additionally, the detection of SCV isolates from herbaceous hosts and strawberry plant material was possible through use of a newly developed gel-based RT-PCR and a gel-free AmpliDet RNA assay. Both methods have the potential to provide rapid, sensitive and specific detection of SCV in in vitro propagation material
    Original languageEnglish
    Pages (from-to)45-52
    JournalEuropean Journal of Plant Pathology
    Volume110
    Issue number1
    DOIs
    Publication statusPublished - 2004

    Keywords

    • multiplex amplidet rna
    • sensitive detection
    • molecular beacons
    • amplification
    • polymerases
    • nasba
    • probes
    • gene

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