Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction

P. Bonants, M. Hagenaar-de Weerdt, M. van Gent-Pelzer, I. Lacourt, D. Cooke, J. Duncan

    Research output: Contribution to journalArticleAcademicpeer-review

    139 Citations (Scopus)

    Abstract

    Phytophthora fragariae Hickman, which causes strawberry red stele and raspberry root rot, is a quarantine organism for which specific and sensitive detection methods are required to test the health of planting material. Sequences of the internal transcribed spacer regions of the ribosomal gene repeat (rDNA) were used to develop primers for P. fragariae in a nested Polymerase Chain Reaction (PCR). The fungus was readily detected in infected but symptomless roots by nested, but not single-round, PCR. It was also detected in infested water samples obtained from the Dutch General Inspection Service by nested PCR. Detection of PCR products was at least 10-fold more sensitive by PCR-ELISA than by conventional visualisation on agarose gels.
    Original languageEnglish
    Pages (from-to)345-355
    JournalEuropean Journal of Plant Pathology
    Volume103
    DOIs
    Publication statusPublished - 1997

    Keywords

    • internal transcribed spacer
    • ITS
    • nested PCR
    • PCR-ELISA
    • Phytophthora
    • ribosomal DNA

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