Detailed mapping of spatial distribution patterns of terrestrial nematodes at landscape scale reveals distinct, ecologically relevant patterns

Research output: Contribution to conferenceAbstractAcademic

Abstract

Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for mainly technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of relatively large molecular frameworks, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. Quantitative (q) PCR assays were used to explore spatial distribution patterns of 45 individual taxa (at family, genus and species level) across the Netherlands at mesoscale (plot or field – scale dimensions: 1 – 1,000 m). Fields were located in two systems (nine arable fields and three grasslands with high plant diversity) and three soil types (marine clay, river clay and sand). In each field 100 composite samples were collected by using a sampling grid (100 x 100m) optimized for geostatistic analysis. Each sample (n >1,200) was analyzed with about 25 - 30 nematode taxon specific molecular assays. In total over 35,000 qPCR reactions were run. Spatial patterns of nematode taxa were mapped by using Integrated Nested Laplace Approximation (INLA) in combination with geostatistical modeling. Patchiness might be related to either nematode traits, such as feeding strategy, body size and colonizer-persister (c-p) scale, or the characteristics of the environment, such as system, soil type and other abiotic conditions. The results that will be presented show a great variation in distribution patterns among nematode taxa, not only on the basis of their traits but also based on environmental characteristics. These data are essential for the design of scientifically sound sampling schemes for nematodes in agricultural and natural soil at hectare scale and above.

Conference

Conference32nd Symposium European Society of Nematologist
CountryPortugal
CityBraga
Period28/08/161/09/16
Internet address

Fingerprint

Nematoda
spatial distribution
soil types
assays
clay
sampling
feeding methods
geostatistics
quantitative analysis
soil quality
Netherlands
labor
quantitative polymerase chain reaction
body size
grasslands
sand
sediments
rivers
DNA
soil

Cite this

@conference{87071c6d6cff4918af8b8166207f3ed3,
title = "Detailed mapping of spatial distribution patterns of terrestrial nematodes at landscape scale reveals distinct, ecologically relevant patterns",
abstract = "Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for mainly technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of relatively large molecular frameworks, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. Quantitative (q) PCR assays were used to explore spatial distribution patterns of 45 individual taxa (at family, genus and species level) across the Netherlands at mesoscale (plot or field – scale dimensions: 1 – 1,000 m). Fields were located in two systems (nine arable fields and three grasslands with high plant diversity) and three soil types (marine clay, river clay and sand). In each field 100 composite samples were collected by using a sampling grid (100 x 100m) optimized for geostatistic analysis. Each sample (n >1,200) was analyzed with about 25 - 30 nematode taxon specific molecular assays. In total over 35,000 qPCR reactions were run. Spatial patterns of nematode taxa were mapped by using Integrated Nested Laplace Approximation (INLA) in combination with geostatistical modeling. Patchiness might be related to either nematode traits, such as feeding strategy, body size and colonizer-persister (c-p) scale, or the characteristics of the environment, such as system, soil type and other abiotic conditions. The results that will be presented show a great variation in distribution patterns among nematode taxa, not only on the basis of their traits but also based on environmental characteristics. These data are essential for the design of scientifically sound sampling schemes for nematodes in agricultural and natural soil at hectare scale and above.",
author = "C.W. Quist and G. Gort and P.J.W. Mooijman and {van den Elsen}, S.J.J. and D.J. Brus and J. Bakker and J. Helder",
year = "2016",
language = "English",
pages = "115--115",
note = "32nd Symposium European Society of Nematologist ; Conference date: 28-08-2016 Through 01-09-2016",
url = "http://esn2016braga.com/",

}

Detailed mapping of spatial distribution patterns of terrestrial nematodes at landscape scale reveals distinct, ecologically relevant patterns. / Quist, C.W.; Gort, G.; Mooijman, P.J.W.; van den Elsen, S.J.J.; Brus, D.J.; Bakker, J.; Helder, J.

2016. 115-115 Abstract from 32nd Symposium European Society of Nematologist, Braga, Portugal.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Detailed mapping of spatial distribution patterns of terrestrial nematodes at landscape scale reveals distinct, ecologically relevant patterns

AU - Quist, C.W.

AU - Gort, G.

AU - Mooijman, P.J.W.

AU - van den Elsen, S.J.J.

AU - Brus, D.J.

AU - Bakker, J.

AU - Helder, J.

PY - 2016

Y1 - 2016

N2 - Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for mainly technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of relatively large molecular frameworks, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. Quantitative (q) PCR assays were used to explore spatial distribution patterns of 45 individual taxa (at family, genus and species level) across the Netherlands at mesoscale (plot or field – scale dimensions: 1 – 1,000 m). Fields were located in two systems (nine arable fields and three grasslands with high plant diversity) and three soil types (marine clay, river clay and sand). In each field 100 composite samples were collected by using a sampling grid (100 x 100m) optimized for geostatistic analysis. Each sample (n >1,200) was analyzed with about 25 - 30 nematode taxon specific molecular assays. In total over 35,000 qPCR reactions were run. Spatial patterns of nematode taxa were mapped by using Integrated Nested Laplace Approximation (INLA) in combination with geostatistical modeling. Patchiness might be related to either nematode traits, such as feeding strategy, body size and colonizer-persister (c-p) scale, or the characteristics of the environment, such as system, soil type and other abiotic conditions. The results that will be presented show a great variation in distribution patterns among nematode taxa, not only on the basis of their traits but also based on environmental characteristics. These data are essential for the design of scientifically sound sampling schemes for nematodes in agricultural and natural soil at hectare scale and above.

AB - Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for mainly technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of relatively large molecular frameworks, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. Quantitative (q) PCR assays were used to explore spatial distribution patterns of 45 individual taxa (at family, genus and species level) across the Netherlands at mesoscale (plot or field – scale dimensions: 1 – 1,000 m). Fields were located in two systems (nine arable fields and three grasslands with high plant diversity) and three soil types (marine clay, river clay and sand). In each field 100 composite samples were collected by using a sampling grid (100 x 100m) optimized for geostatistic analysis. Each sample (n >1,200) was analyzed with about 25 - 30 nematode taxon specific molecular assays. In total over 35,000 qPCR reactions were run. Spatial patterns of nematode taxa were mapped by using Integrated Nested Laplace Approximation (INLA) in combination with geostatistical modeling. Patchiness might be related to either nematode traits, such as feeding strategy, body size and colonizer-persister (c-p) scale, or the characteristics of the environment, such as system, soil type and other abiotic conditions. The results that will be presented show a great variation in distribution patterns among nematode taxa, not only on the basis of their traits but also based on environmental characteristics. These data are essential for the design of scientifically sound sampling schemes for nematodes in agricultural and natural soil at hectare scale and above.

M3 - Abstract

SP - 115

EP - 115

ER -