Detailed biochemical and morphologic characteristics of the green microalga Neochloris oleoabundans cell wall

Behzad Rashidi, Luisa M. Trindade*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Chlorophyta, the group of green algae of which there are >6000 species, manifests a great diversity of intercellular and extracellular components. Building blocks in the cell walls of Chlorophyta are very distinct and they may contain various components. Here, we characterize the cell walls of Neochloris oleoabundans, a Chlorophyte microalga, both in terms of biochemical composition and morphology. N. oleoabundans cell walls are composed of about 24.3% carbohydrates, 31.5% proteins, 22.2% lipids and 7.8% inorganic material, which contrasts to the cell walls of (higher) terrestrial plants in which carbohydrates are by far the main component. We also observed that cell wall carbohydrates are mainly non-cellulosic polysaccharides, essentially composed of rhamnose, galactose, glucuronic acid and glucosamine, of which glucose is only a minor component. The lipids comprising the N. oleoabundans cell walls are generally wax/cutin-like. Electron microscopic studies revealed that N. oleabundans cell walls are approximately 200 nm thick and consist of two main layers: a thinner inner layer and a more electron-dense outer layer. On the outer layer are hair-like structures that are possibly rich in carbohydrates. These findings are an important contribution that enable us to understand the complexity of cell walls in green microalgae.

Original languageEnglish
Pages (from-to)152-159
JournalAlgal Research
Volume35
DOIs
Publication statusPublished - 1 Nov 2018

Fingerprint

Ettlia oleoabundans
cell walls
carbohydrates
Chlorophyta
electrons
cutin
glucuronic acid
rhamnose
glucosamine
lipids
microalgae
waxes
galactose
trichomes
polysaccharides
chemical composition

Keywords

  • Biochemical composition
  • Cell wall
  • Chlorophyta
  • Electron microscopy
  • Green microalgae
  • Neochloris oleoabundans

Cite this

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title = "Detailed biochemical and morphologic characteristics of the green microalga Neochloris oleoabundans cell wall",
abstract = "Chlorophyta, the group of green algae of which there are >6000 species, manifests a great diversity of intercellular and extracellular components. Building blocks in the cell walls of Chlorophyta are very distinct and they may contain various components. Here, we characterize the cell walls of Neochloris oleoabundans, a Chlorophyte microalga, both in terms of biochemical composition and morphology. N. oleoabundans cell walls are composed of about 24.3{\%} carbohydrates, 31.5{\%} proteins, 22.2{\%} lipids and 7.8{\%} inorganic material, which contrasts to the cell walls of (higher) terrestrial plants in which carbohydrates are by far the main component. We also observed that cell wall carbohydrates are mainly non-cellulosic polysaccharides, essentially composed of rhamnose, galactose, glucuronic acid and glucosamine, of which glucose is only a minor component. The lipids comprising the N. oleoabundans cell walls are generally wax/cutin-like. Electron microscopic studies revealed that N. oleabundans cell walls are approximately 200 nm thick and consist of two main layers: a thinner inner layer and a more electron-dense outer layer. On the outer layer are hair-like structures that are possibly rich in carbohydrates. These findings are an important contribution that enable us to understand the complexity of cell walls in green microalgae.",
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Detailed biochemical and morphologic characteristics of the green microalga Neochloris oleoabundans cell wall. / Rashidi, Behzad; Trindade, Luisa M.

In: Algal Research, Vol. 35, 01.11.2018, p. 152-159.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Detailed biochemical and morphologic characteristics of the green microalga Neochloris oleoabundans cell wall

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AU - Trindade, Luisa M.

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N2 - Chlorophyta, the group of green algae of which there are >6000 species, manifests a great diversity of intercellular and extracellular components. Building blocks in the cell walls of Chlorophyta are very distinct and they may contain various components. Here, we characterize the cell walls of Neochloris oleoabundans, a Chlorophyte microalga, both in terms of biochemical composition and morphology. N. oleoabundans cell walls are composed of about 24.3% carbohydrates, 31.5% proteins, 22.2% lipids and 7.8% inorganic material, which contrasts to the cell walls of (higher) terrestrial plants in which carbohydrates are by far the main component. We also observed that cell wall carbohydrates are mainly non-cellulosic polysaccharides, essentially composed of rhamnose, galactose, glucuronic acid and glucosamine, of which glucose is only a minor component. The lipids comprising the N. oleoabundans cell walls are generally wax/cutin-like. Electron microscopic studies revealed that N. oleabundans cell walls are approximately 200 nm thick and consist of two main layers: a thinner inner layer and a more electron-dense outer layer. On the outer layer are hair-like structures that are possibly rich in carbohydrates. These findings are an important contribution that enable us to understand the complexity of cell walls in green microalgae.

AB - Chlorophyta, the group of green algae of which there are >6000 species, manifests a great diversity of intercellular and extracellular components. Building blocks in the cell walls of Chlorophyta are very distinct and they may contain various components. Here, we characterize the cell walls of Neochloris oleoabundans, a Chlorophyte microalga, both in terms of biochemical composition and morphology. N. oleoabundans cell walls are composed of about 24.3% carbohydrates, 31.5% proteins, 22.2% lipids and 7.8% inorganic material, which contrasts to the cell walls of (higher) terrestrial plants in which carbohydrates are by far the main component. We also observed that cell wall carbohydrates are mainly non-cellulosic polysaccharides, essentially composed of rhamnose, galactose, glucuronic acid and glucosamine, of which glucose is only a minor component. The lipids comprising the N. oleoabundans cell walls are generally wax/cutin-like. Electron microscopic studies revealed that N. oleabundans cell walls are approximately 200 nm thick and consist of two main layers: a thinner inner layer and a more electron-dense outer layer. On the outer layer are hair-like structures that are possibly rich in carbohydrates. These findings are an important contribution that enable us to understand the complexity of cell walls in green microalgae.

KW - Biochemical composition

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