Design strategies for integrated protein purification processes: challenges, progress and outlook

B. Nfor, T. Ahamed, G. Dedem, L. van der Wielen, E. van de Sandt, M.H.M. Eppink, M. Ottens

Research output: Contribution to journalArticleAcademicpeer-review

67 Citations (Scopus)

Abstract

The key to successful and efficient protein purification is the selection of the most appropriate purification techniques and their combination in a logical way to obtain the desired purification in the minimum number of steps. However, the rationalization of protein purification process development is faced with a number of challenges. In this paper, the challenges in protein purification process development are captured. The state-of-the-art in protein purification process synthesis and design is reviewed and the strengths and weaknesses of the current strategies highlighted. Finally, views on the future directions of protein purification process development are presented
Original languageEnglish
Pages (from-to)124-132
JournalJournal of Chemical Technology and Biotechnology
Volume83
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • expert-system
  • physicochemical properties
  • exchange chromatography
  • biochemical processes
  • recombinant proteins
  • purify proteins
  • optimization
  • selection
  • bioseparations
  • bioprocesses

Fingerprint

Dive into the research topics of 'Design strategies for integrated protein purification processes: challenges, progress and outlook'. Together they form a unique fingerprint.

Cite this