Design of a confocal microfluidic particle sorter using fluorescent photon burst detection

B.H. Kunst, A. Schots, A.J.W.G. Visser

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

An instrumental system is described for detecting and sorting single fluorescent particles such as microspheres, bacteria, viruses, or even smaller macromolecules in a flowing liquid. The system consists of microfluidic chips (biochips), computer controlled high voltage power supplies, and a fluorescence microscope with confocal optics. The confocal observation volume and detection electro-optics allow measurements of single flowing fluorescent particles. The output of the avalanche photodiode (single photon detector) is coupled to a real-time photon-burst detection device, which output can address the control of high voltage power supplies for sorting purposes. Liquid propulsion systems like electro-osmotic flow and plain electric fields to direct the particles through the observation volume have been tested and evaluated. The detection and real-time sorting of fluorescent microspheres are demonstrated. Applications of these biochips for screening of bacteriophages-type biolibraries are briefly discussed. (C) 2004 American Institute of Physics.
Original languageEnglish
Pages (from-to)2892-2898
JournalReview of Scientific Instruments
Volume75
Issue number9
DOIs
Publication statusPublished - 2004

Keywords

  • single-molecule detection
  • correlation spectroscopy
  • submicrometer channels
  • cross-correlation
  • fluidic channels
  • flow-cytometry
  • cell sorter
  • dna
  • microcapillary
  • confinement

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