Design and development of a DNA array for rapid detection and identification of tomato vascular wilt pathogens

B. Lievens, M. Brouwer, A.C.R.C. Vanachter, C.A. Lévesque, B.P.A. Cammue, B.P.H.J. Thomma

Research output: Contribution to journalArticleAcademicpeer-review

103 Citations (Scopus)

Abstract

Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, and Verticillium wilt, caused by either Verticillium albo-atrum or Verticillium dahliae, are devastating diseases of tomato (Lycopersicon esculentum) found worldwide. Monitoring is the cornerstone of integrated pest management of any disease. The lack of rapid, accurate, and reliable means by which plant pathogens can be detected and identified is one of the main limitations in integrated disease management. In this paper, we describe the development of a molecular detection system, based on DNA array technology, for rapid and efficient detection of these vascular wilt pathogens. We show the utility of this array for the sensitive detection of these pathogens from complex substrates like soil, plant tissues and irrigation water, and samples that are collected by tomato growers in their greenhouses.
Original languageEnglish
Pages (from-to)113-122
JournalFEMS Microbiology Letters
Volume223
Issue number1
DOIs
Publication statusPublished - 2003

Keywords

  • verticillium-albo-atrum
  • dot-blot hybridization
  • f-sp lycopersici
  • nonpathogenic fusarium-oxysporum
  • biological-control
  • genetic-analysis
  • dahliae
  • microsclerotia
  • combination
  • resistance

Fingerprint Dive into the research topics of 'Design and development of a DNA array for rapid detection and identification of tomato vascular wilt pathogens'. Together they form a unique fingerprint.

  • Cite this