Depletion - flocculation in oil-in-water emulsions using fibrillar protein assemblies

T.B.J. Blijdenstein, C. Veerman, E. van der Linden

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)


This paper shows that low concentrations of -lactoglobulin fibrils can induce depletion-flocculation in -lactoglobulin-stabilized oil-in-water emulsions. The minimum required fibril concentration for flocculation was determined experimentally for fibril lengths of about 3 and 0.1 m. The minimum fibril concentration for flocculation is two orders of magnitude higher for the short fibrils than for the long ones. These experimental results correspond well with a theoretical prediction based on a model of spinodal decomposition. In addition, rheological measurements were performed, verifying that flocculation was induced by a depletion mechanism. The results of this study show that the minimum concentration required for depletion-flocculation can be tuned by varying the length of the fibrils.
Original languageEnglish
Pages (from-to)4881-4884
Issue number12
Publication statusPublished - 2004


  • beta-lactoglobulin
  • dextran sulfate
  • gels
  • interfaces
  • stability

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