Demonstration of In vivo expression of a hypothetical open reading frame (ORF-14) encoded by the RD1 region of Mycobacterium tuberculosis

H.A. Amoudy, S. Ahmad, J.E.R. Thole, A.S. Mustafa

    Research output: Contribution to journalArticleAcademicpeer-review

    14 Citations (Scopus)

    Abstract

    Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics.
    Original languageEnglish
    Pages (from-to)422-425
    JournalScandinavian Journal of Immunology
    Volume66
    Issue number4
    DOIs
    Publication statusPublished - 2007

    Keywords

    • t-cell responses
    • calmette-guerin
    • genome sequence
    • esat-6 antigen
    • identification
    • proteins
    • epitopes
    • bcg
    • immunogenicity
    • compilation

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