Delayed effects of environmentally relevant concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB-77) and non-polar sediment extracts detected in the prolonged-FETAX

A.C. Gutleb, L. Mossink, M. Schriks, J.H.J. van den Berg, A.J. Murk

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

In the prolonged-FETAX (prolonged-Frog Embryo Teratogenic Assay-Xenopus) tadpoles are allowed to develop until metamorphosis after an initial 4 day early life-stage exposure (FETAX). PCB 77 (3,4,3¿,4¿-tetrachlorobiphenyl) and sediment extracts were used in the presented experiments. Concentrations of PCB 77 (0.03 nM = 8.55 ng/L; 3 nM = 855 ng/L; 300 nM = 85.5 ¿g/L, 30,000 nM = 8.55 mg/L) were derived on the basis of dioxin-like toxic equivalency concentrations known to be present in pore-water. The results were compared to those obtained with the standard FETAX. In the prolonged-FETAX the percentage of animals passing metamorphosis within 115 days was significantly decreased in the groups exposed to 300 or 30,000 nM PCB 77. Significantly increased percentages of tadpoles were halted in thyroid hormone dependent early metamorphic NF stages ¿ 55 (3 nM PCB 77) and metamorphic stages 56¿60 tadpoles (300 nM PCB 77 and 30,000 nM PCB 77). Non-polar sediment extracts from two contaminated Dutch sites significantly decreased, and one significantly increased the percentage of animals that passed metamorphosis. These results were in accordance with earlier in vitro results in a thyroid hormone dependent assay and strongly suggest the presence of thyroid hormone disrupting compounds in the aquatic environment. In the classical FETAX neither PCB 77 nor non-polar sediment extracts induced any effects. The results of the present study indicate that the prolonged-FETAX is an important in vivo tool in addition to the in vitro T-screen to assess long-term effects of early life-stage exposure to environmentally relevant concentrations of (mixtures of) compounds and that affect thyroid hormone dependent physiology.
Original languageEnglish
Pages (from-to)307-315
Number of pages9
JournalScience of the Total Environment
Volume381
Issue number1-3
DOIs
Publication statusPublished - 2007

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3,4,3',4'-tetrachlorobiphenyl
Polychlorinated biphenyls
frog
embryo
PCB
Assays
Sediments
assay
Thyroid Hormones
hormone
sediment
metamorphosis
Animals
Dioxins
animal
Poisons
Physiology
effect
dioxin
aquatic environment

Keywords

  • embryo teratogenesis assay
  • metabolic-activation system
  • receptor active compounds
  • species xenopus-laevis
  • thyroid-hormone
  • developmental toxicity
  • gene-expression
  • rana-temporaria
  • polychlorinated-biphenyls
  • amphibian metamorphosis

Cite this

@article{4a2fe919195b468cbdbb91dd4e53af94,
title = "Delayed effects of environmentally relevant concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB-77) and non-polar sediment extracts detected in the prolonged-FETAX",
abstract = "In the prolonged-FETAX (prolonged-Frog Embryo Teratogenic Assay-Xenopus) tadpoles are allowed to develop until metamorphosis after an initial 4 day early life-stage exposure (FETAX). PCB 77 (3,4,3¿,4¿-tetrachlorobiphenyl) and sediment extracts were used in the presented experiments. Concentrations of PCB 77 (0.03 nM = 8.55 ng/L; 3 nM = 855 ng/L; 300 nM = 85.5 ¿g/L, 30,000 nM = 8.55 mg/L) were derived on the basis of dioxin-like toxic equivalency concentrations known to be present in pore-water. The results were compared to those obtained with the standard FETAX. In the prolonged-FETAX the percentage of animals passing metamorphosis within 115 days was significantly decreased in the groups exposed to 300 or 30,000 nM PCB 77. Significantly increased percentages of tadpoles were halted in thyroid hormone dependent early metamorphic NF stages ¿ 55 (3 nM PCB 77) and metamorphic stages 56¿60 tadpoles (300 nM PCB 77 and 30,000 nM PCB 77). Non-polar sediment extracts from two contaminated Dutch sites significantly decreased, and one significantly increased the percentage of animals that passed metamorphosis. These results were in accordance with earlier in vitro results in a thyroid hormone dependent assay and strongly suggest the presence of thyroid hormone disrupting compounds in the aquatic environment. In the classical FETAX neither PCB 77 nor non-polar sediment extracts induced any effects. The results of the present study indicate that the prolonged-FETAX is an important in vivo tool in addition to the in vitro T-screen to assess long-term effects of early life-stage exposure to environmentally relevant concentrations of (mixtures of) compounds and that affect thyroid hormone dependent physiology.",
keywords = "embryo teratogenesis assay, metabolic-activation system, receptor active compounds, species xenopus-laevis, thyroid-hormone, developmental toxicity, gene-expression, rana-temporaria, polychlorinated-biphenyls, amphibian metamorphosis",
author = "A.C. Gutleb and L. Mossink and M. Schriks and {van den Berg}, J.H.J. and A.J. Murk",
year = "2007",
doi = "10.1016/j.scitotenv.2007.03.002",
language = "English",
volume = "381",
pages = "307--315",
journal = "Science of the Total Environment",
issn = "0048-9697",
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}

Delayed effects of environmentally relevant concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB-77) and non-polar sediment extracts detected in the prolonged-FETAX. / Gutleb, A.C.; Mossink, L.; Schriks, M.; van den Berg, J.H.J.; Murk, A.J.

In: Science of the Total Environment, Vol. 381, No. 1-3, 2007, p. 307-315.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Delayed effects of environmentally relevant concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB-77) and non-polar sediment extracts detected in the prolonged-FETAX

AU - Gutleb, A.C.

AU - Mossink, L.

AU - Schriks, M.

AU - van den Berg, J.H.J.

AU - Murk, A.J.

PY - 2007

Y1 - 2007

N2 - In the prolonged-FETAX (prolonged-Frog Embryo Teratogenic Assay-Xenopus) tadpoles are allowed to develop until metamorphosis after an initial 4 day early life-stage exposure (FETAX). PCB 77 (3,4,3¿,4¿-tetrachlorobiphenyl) and sediment extracts were used in the presented experiments. Concentrations of PCB 77 (0.03 nM = 8.55 ng/L; 3 nM = 855 ng/L; 300 nM = 85.5 ¿g/L, 30,000 nM = 8.55 mg/L) were derived on the basis of dioxin-like toxic equivalency concentrations known to be present in pore-water. The results were compared to those obtained with the standard FETAX. In the prolonged-FETAX the percentage of animals passing metamorphosis within 115 days was significantly decreased in the groups exposed to 300 or 30,000 nM PCB 77. Significantly increased percentages of tadpoles were halted in thyroid hormone dependent early metamorphic NF stages ¿ 55 (3 nM PCB 77) and metamorphic stages 56¿60 tadpoles (300 nM PCB 77 and 30,000 nM PCB 77). Non-polar sediment extracts from two contaminated Dutch sites significantly decreased, and one significantly increased the percentage of animals that passed metamorphosis. These results were in accordance with earlier in vitro results in a thyroid hormone dependent assay and strongly suggest the presence of thyroid hormone disrupting compounds in the aquatic environment. In the classical FETAX neither PCB 77 nor non-polar sediment extracts induced any effects. The results of the present study indicate that the prolonged-FETAX is an important in vivo tool in addition to the in vitro T-screen to assess long-term effects of early life-stage exposure to environmentally relevant concentrations of (mixtures of) compounds and that affect thyroid hormone dependent physiology.

AB - In the prolonged-FETAX (prolonged-Frog Embryo Teratogenic Assay-Xenopus) tadpoles are allowed to develop until metamorphosis after an initial 4 day early life-stage exposure (FETAX). PCB 77 (3,4,3¿,4¿-tetrachlorobiphenyl) and sediment extracts were used in the presented experiments. Concentrations of PCB 77 (0.03 nM = 8.55 ng/L; 3 nM = 855 ng/L; 300 nM = 85.5 ¿g/L, 30,000 nM = 8.55 mg/L) were derived on the basis of dioxin-like toxic equivalency concentrations known to be present in pore-water. The results were compared to those obtained with the standard FETAX. In the prolonged-FETAX the percentage of animals passing metamorphosis within 115 days was significantly decreased in the groups exposed to 300 or 30,000 nM PCB 77. Significantly increased percentages of tadpoles were halted in thyroid hormone dependent early metamorphic NF stages ¿ 55 (3 nM PCB 77) and metamorphic stages 56¿60 tadpoles (300 nM PCB 77 and 30,000 nM PCB 77). Non-polar sediment extracts from two contaminated Dutch sites significantly decreased, and one significantly increased the percentage of animals that passed metamorphosis. These results were in accordance with earlier in vitro results in a thyroid hormone dependent assay and strongly suggest the presence of thyroid hormone disrupting compounds in the aquatic environment. In the classical FETAX neither PCB 77 nor non-polar sediment extracts induced any effects. The results of the present study indicate that the prolonged-FETAX is an important in vivo tool in addition to the in vitro T-screen to assess long-term effects of early life-stage exposure to environmentally relevant concentrations of (mixtures of) compounds and that affect thyroid hormone dependent physiology.

KW - embryo teratogenesis assay

KW - metabolic-activation system

KW - receptor active compounds

KW - species xenopus-laevis

KW - thyroid-hormone

KW - developmental toxicity

KW - gene-expression

KW - rana-temporaria

KW - polychlorinated-biphenyls

KW - amphibian metamorphosis

U2 - 10.1016/j.scitotenv.2007.03.002

DO - 10.1016/j.scitotenv.2007.03.002

M3 - Article

VL - 381

SP - 307

EP - 315

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

IS - 1-3

ER -