Delay of germination 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination

Kai Graeber, Ada Linkies, Tina Steinbrecher, Klaus Mummenhoff, Danuše Tarkowská, Veronika Turečková, Michael Ignatz, Katja Sperber, Antje Voegele, Hans De Jong, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

87 Citations (Scopus)

Abstract

Seed germination is an important life-cycle transition because it determines subsequent plant survival and reproductive success. To detect optimal spatiotemporal conditions for germination, seeds act as sophisticated environmental sensors integrating information such as ambient temperature. Here we show that the DELAY OF GERMINATION 1 (DOG1) gene, known for providing dormancy adaptation to distinct environments, determines the optimal temperature for seed germination. By reciprocal gene-swapping experiments between Brassicaceae species we show that the DOG1-mediated dormancy mechanism is conserved. Biomechanical analyses showthat thismechanism regulates the material properties of the endosperm, a seed tissue layer acting as germination barrier to control coat dormancy. We found that DOG1 inhibits the expression of gibberellin (GA)-regulated genes encoding cell-wall remodeling proteins in a temperature-dependent manner. Furthermore we demonstrate that DOG1 causes temperature-dependent alterations in the seed GA metabolism. These alterations in hormone metabolism are brought about by the temperature-dependent differential expression of genes encoding key enzymes of the GA biosynthetic pathway. These effects of DOG1 lead to a temperature-dependent control of endosperm weakening and determine the optimal temperature for germination. The conserved DOG1-mediated coat-dormancymechanismprovides a highly adaptable temperature-sensing mechanism to control the timing of germination.

Original languageEnglish
Pages (from-to)E3571-E3580
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number34
DOIs
Publication statusPublished - 26 Aug 2014

Keywords

  • Cell-wall remodelling
  • Dormancy gene DOG1
  • Germination temperature
  • Gibberellin metabolism
  • Lepidium sativum

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    Graeber, K., Linkies, A., Steinbrecher, T., Mummenhoff, K., Tarkowská, D., Turečková, V., Ignatz, M., Sperber, K., Voegele, A., De Jong, H., Urbanová, T., Strnad, M., & Leubner-Metzger, G. (2014). Delay of germination 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. Proceedings of the National Academy of Sciences of the United States of America, 111(34), E3571-E3580. https://doi.org/10.1073/pnas.1403851111