Degradation of 3,4-dichloro- and 3,4-difluoroaniline by Pseudomonas fluorescens 26K

V. Travkin, I.P. Solyanikova, I.M.C.M. Rietjens, J.J.M. Vervoort, W.J.H. van Berkel, L.A. Golovleva

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43 Citations (Scopus)

Abstract

3,4-Dichloro- and 3,4-difluoroanilines were degraded by Pseudomonas fluorescens 26-K under aerobic conditions. In the presence of glucose strain degraded 170 mg/L of 3,4-dichloroaniline (3,4-DCA) during 2-3 days. Increasing of toxicant concentration up to 250 mg/L led to degradation of 3,4-DCA during 4 days and its intermediates during 5-7 days. Without cosubstrate and nitrogen source degradation of 3,4-DCA took place too, but more slowly-about 40% of toxicant at initial concentration 75 mg/L was degraded during 15 days. 3,4-Difluoroaniline (3,4-DFA) (initial concentration 170 mg/L) was degraded by Pseudontonas fluorescens 26-K during 5-7 days. The strain was able to completely degrade up to 90 mg/L of 3,4-DFA, without addition of cosubstrate and nitrogen during 15 days. Degradation of fluorinated aniline was accompanied by intensive defluorination. Activity of catechol 2,3-dioxygenase (C2,3DO) (0.230 mumol/min/mg of protein) was found in the culture liquid of the strain, grown with 3,4-DCA and glucose. This fact, as well as, the presence of 3-chloro-4-hydroxyaniline as a metabolite suggested that 3,4-DCA degradation pathway includes dehalogenation and hydroxylation of aromatic ring followed by its subsequent cleaving by C2,3DO. On the contrary, activity of catechol 1,2-dioxygenase (C1,2DO) (0.08 mumol/min/mg of protein) was found in the cell-free extract of biomass grown on 3,4-DFA. 3 -Fluoro-4-hydroxyani line as intermediate was found in this cell-free extract.
Original languageEnglish
Pages (from-to)121-132
JournalJournal of Environmental Science and Health. Part B, Pesticides Food Contaminants, and agricultural wastes
Volume38
DOIs
Publication statusPublished - 2003

Keywords

  • microbial-degradation
  • aniline
  • chloroanilines
  • soil

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