Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production

Li Hua Zhu*, Frans Krens, Mark A. Smith, Xueyuan Li, Weicong Qi, Eibertus N. Van Loo, Tim Iven, Ivo Feussner, Tara J. Nazarenus, Dongxin Huai, David C. Taylor, Xue Rong Zhou, Allan G. Green, Jay Shockey, Thomas K. Klasson, Robert T. Mullen, Bangquan Huang, John M. Dyer, Edgar B. Cahoon

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.

Original languageEnglish
Article number22181
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016

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wax esters
metabolic engineering
oilseed crops
oilseeds
feedstocks
industrial oils
Crambe (Brassicaceae)
lubricants
vegetable oil
nonrenewable resources
Camelina
fatty acids
petrochemicals
industrial applications
oxidative stability
crops
Brassicaceae
seed oils
petroleum
biotechnology

Cite this

Zhu, Li Hua ; Krens, Frans ; Smith, Mark A. ; Li, Xueyuan ; Qi, Weicong ; Van Loo, Eibertus N. ; Iven, Tim ; Feussner, Ivo ; Nazarenus, Tara J. ; Huai, Dongxin ; Taylor, David C. ; Zhou, Xue Rong ; Green, Allan G. ; Shockey, Jay ; Klasson, Thomas K. ; Mullen, Robert T. ; Huang, Bangquan ; Dyer, John M. ; Cahoon, Edgar B. / Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production. In: Scientific Reports. 2016 ; Vol. 6.
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title = "Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production",
abstract = "Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20{\%} of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.",
author = "Zhu, {Li Hua} and Frans Krens and Smith, {Mark A.} and Xueyuan Li and Weicong Qi and {Van Loo}, {Eibertus N.} and Tim Iven and Ivo Feussner and Nazarenus, {Tara J.} and Dongxin Huai and Taylor, {David C.} and Zhou, {Xue Rong} and Green, {Allan G.} and Jay Shockey and Klasson, {Thomas K.} and Mullen, {Robert T.} and Bangquan Huang and Dyer, {John M.} and Cahoon, {Edgar B.}",
year = "2016",
doi = "10.1038/srep22181",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

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Zhu, LH, Krens, F, Smith, MA, Li, X, Qi, W, Van Loo, EN, Iven, T, Feussner, I, Nazarenus, TJ, Huai, D, Taylor, DC, Zhou, XR, Green, AG, Shockey, J, Klasson, TK, Mullen, RT, Huang, B, Dyer, JM & Cahoon, EB 2016, 'Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production', Scientific Reports, vol. 6, 22181. https://doi.org/10.1038/srep22181

Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production. / Zhu, Li Hua; Krens, Frans; Smith, Mark A.; Li, Xueyuan; Qi, Weicong; Van Loo, Eibertus N.; Iven, Tim; Feussner, Ivo; Nazarenus, Tara J.; Huai, Dongxin; Taylor, David C.; Zhou, Xue Rong; Green, Allan G.; Shockey, Jay; Klasson, Thomas K.; Mullen, Robert T.; Huang, Bangquan; Dyer, John M.; Cahoon, Edgar B.

In: Scientific Reports, Vol. 6, 22181, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production

AU - Zhu, Li Hua

AU - Krens, Frans

AU - Smith, Mark A.

AU - Li, Xueyuan

AU - Qi, Weicong

AU - Van Loo, Eibertus N.

AU - Iven, Tim

AU - Feussner, Ivo

AU - Nazarenus, Tara J.

AU - Huai, Dongxin

AU - Taylor, David C.

AU - Zhou, Xue Rong

AU - Green, Allan G.

AU - Shockey, Jay

AU - Klasson, Thomas K.

AU - Mullen, Robert T.

AU - Huang, Bangquan

AU - Dyer, John M.

AU - Cahoon, Edgar B.

PY - 2016

Y1 - 2016

N2 - Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.

AB - Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.

U2 - 10.1038/srep22181

DO - 10.1038/srep22181

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 22181

ER -