Deconjugation of soy isoflavone glucuronides needed for estrogenic activity

M.A. Islam, R. Bekele, J.H.J. van den Berg, Y. Kuswanti, O. Thapa, S. Soltani, F.X.R. Leeuwen, I.M.C.M. Rietjens, A.J. Murk

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)

Abstract

Soy isoflavones (SIF) are present in the systemic circulation as conjugated forms of which the estrogenic potency is not yet clear. The present study provides evidence that the major SIF glucuronide metabolites in blood, genistein-7-O-glucuronide (GG) and daidzein-7-O-glucuronide (DG), only become estrogenic after deconjugation. The estrogenic potencies of genistein (Ge), daidzein (Da), GG and DG were determined using stably transfected U2OS-ERa, U2OS-ERß reporter gene cells and proliferation was tested in T47D-ERß cells mimicking the ERa/ERß ratio of healthy breast cells and inT47D breast cancer cells. In all assays applied, the estrogenic potency of the aglycones was significantly higher than that of their corresponding glucuronides. UPLC analysis revealed that in U2OS and T47D cells, 0.2-1.6% of the glucuronides were deconjugated to their corresponding aglycones. The resulting aglycone concentrations can account for the estrogenicity observed upon glucuronide exposure. Interestingly, under similar experimental conditions, rat breast tissue S9 fraction was about 30 times more potent in deconjugating these glucuronides than human breast tissue S9 fraction. Our study confirms that SIF glucuronides are not estrogenic as such, and that the small % of deconjugation in the cell is enough to explain the slight bioactivity observed for the SIF-glucuronides. Species differences in deconjugation capacity should be taken into account when basing risk-benefit assessment of these SIF for the human population on animal data.
Original languageEnglish
Pages (from-to)706-715
JournalToxicology in Vitro
Volume29
Issue number4
DOIs
Publication statusPublished - 2015

Keywords

  • beta-messenger-rna
  • in-vitro
  • er-beta
  • receptor-beta
  • cell-proliferation
  • human plasma
  • cancer
  • expression
  • alpha
  • genistein

Fingerprint Dive into the research topics of 'Deconjugation of soy isoflavone glucuronides needed for estrogenic activity'. Together they form a unique fingerprint.

Cite this