Dechloromonas hortensis sp.nov. and strain ASK-1, two novel (per) chlorate-reducing bacteria, and taxonomic description of strain GR-1

A.F.W.M. Wolterink, S. Kim, M. Muusse, I.S. Kim, P.J. Roholl, C.G. van Ginkel, A.J.M. Stams, S.W.M. Kengen

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63 Citations (Scopus)

Abstract

Recent studies on the occurrence of (per)chlorate-reducing bacteria have resulted in the characterization of strains capable of dissimilatory (per)chlorate reduction. Phylogenetic analysis has shown that these bacteria are members of the Proteobacteria. Strains have been isolated from polluted and pristine sites, but only strains from polluted sites have been characterized in detail and deposited in culture collections. Herein we describe the isolation and characterization of perchlorate-reducing bacterium strain MA-1T and chlorate-reducing bacterium strain ASK-1, respectively isolated from a pristine and a chlorate-polluted site. Both isolates are members of the Proteobacteria. The 16S rRNA gene sequence similarity of MA-1T to Dechloromonas agitata DSM 13637T is 97·6 %, but the relatedness in DNA¿DNA reassociation is only 37 %. Therefore, we propose to classify strain MA-1T (=DSM 15637T=ATCC BAA-776T) as the type strain of a novel species, Dechloromonas hortensis sp. nov. Strain ASK-1 and a previously described strain GR-1 show 100 and 99 % 16S rRNA gene sequence similarity to Pseudomonas chloritidismutans DSM 13592T and Dechlorosoma suillum DSM 13638T, respectively. DNA¿DNA hybridization studies indicated that strains ASK-1 and GR-1 are related at the species level to P. chloritidismutans DSM 13592T (79 %) and Dechlorosoma suillum DSM 13638T (85 %), respectively. As suggested previously, Dechlorosoma suillum appears to be a later heterotypic synonym of Azospira oryzae. Although strain ASK-1 is identified as P. chloritidismutans, its morphology and growth requirements are different from those of the type strain
Original languageEnglish
Pages (from-to)2063-2068
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume55
Issue number5
DOIs
Publication statusPublished - 2005

Keywords

  • gen. nov.
  • dna
  • purification
  • renaturation
  • perchlorate
  • reduction
  • chlorate
  • metabolism
  • sediments
  • azoarcus

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