De novo production of the flavonoid naringenin in engineered Saccharomyces erevisiae

F. Koopman, J. Beekwilder, B. Crimi, A. van Houwelingen, R.D. Hall, H.J. Bosch, A.J.A. van Maris, J.T. Pronk, J.M. Daran

Research output: Contribution to journalArticleAcademicpeer-review

182 Citations (Scopus)

Abstract

Background: Flavonoids comprise a large family of secondary plant metabolic intermediates that exhibit a wide variety of antioxidant and human health-related properties. Plant production of flavonoids is limited by the low productivity and the complexity of the recovered flavonoids. Thus to overcome these limitations, metabolic engineering of specific pathway in microbial systems have been envisaged to produce high quantity of a single molecules. Result: Saccharomyces cerevisiae was engineered to produce the key intermediate flavonoid, naringenin, solely from glucose. For this, specific naringenin biosynthesis genes from Arabidopsis thaliana were selected by comparative expression profiling and introduced in S. cerevisiae. The sole expression of these A. thaliana genes yielded low extracellular naringenin concentrations (
Original languageEnglish
Article number155
JournalMicrobial Cell Factories
Volume11
DOIs
Publication statusPublished - 2012

Keywords

  • tyrosine ammonia-lyase
  • escherichia-coli
  • recombinant microorganisms
  • liquid-chromatography
  • cancer-cells
  • in-vitro
  • yeast
  • biosynthesis
  • arabidopsis
  • expression

Fingerprint Dive into the research topics of 'De novo production of the flavonoid naringenin in engineered Saccharomyces erevisiae'. Together they form a unique fingerprint.

  • Cite this