De invloed van auxine, tryptofaan en enige anorganische zouten op de infectie van Nicotiana glutinosa met tabaksmozaiekvirus

A.S.N. Liem

Research output: Thesisinternal PhD, WU

Abstract

The number of necrotic spots arising on leaves of Nicotiana glutinosa after inoculation with tobacco mosaic virus was less than in controls without additives, if the water in which the leaves floated hadβ-indoleacetic acid (IAA),α-naphthylacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) in correct concentrations, added 16 h before (IAA also after) inoculation. This reaction occurred always in weak light but not always in strong light. D-tryptophan, but not DL and L isomers, caused a similar effect in only half the tests. In vitro IAA did not inactivate the virus. IAA, NAA and 2,4-D all decreased the osmotic pressure of the leaf sap of plants in weak light. IAA also decreased the conductivity of the sap of floating leaves by exosmosis. Leaves of plants in strong light had higher osmotic pressure, but if both leaf types were floated on water, the difference in osmotic pressure disappeared. Yet water absorption seemed not to be directly related to the decrease in osmotic pressure with IAA.
A mixture of Ca (NO 3 ) 2 , KNO 3 and MgS0 4 increased osmotic pressure and number of necrotic spots, although less with IAA than without it. Ca 2+increased and K +and Mg 2+decreased number of spots. Perhaps phosphate-buffer enhanced auxin production, so masking the influence of the applied IAA. Probably IAA stimulated synthesis of enzymes and specific nucleic acid, so using up compounds needed for virus synthesis.
Original languageDutch
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • van der Want, J.P.H., Promotor, External person
  • van Raalte, M.H., Promotor, External person
Award date3 May 1963
Place of PublicationWageningen
Publisher
Publication statusPublished - 1963

Keywords

  • tobacco mosaic virus
  • auxins
  • nicotiana glutinosa
  • tryptophan
  • inorganic salts

Cite this

@phdthesis{1b5427807a8a4d6ea44e77deba429526,
title = "De invloed van auxine, tryptofaan en enige anorganische zouten op de infectie van Nicotiana glutinosa met tabaksmozaiekvirus",
keywords = "tabaksmoza{\"i}ekvirus, auxinen, nicotiana glutinosa, tryptofaan, anorganische zouten, tobacco mosaic virus, auxins, nicotiana glutinosa, tryptophan, inorganic salts",
author = "A.S.N. Liem",
note = "WU thesis 345 Proefschrift Wageningen",
year = "1963",
language = "Dutch",
publisher = "Pudoc",
school = "Wageningen University",

}

De invloed van auxine, tryptofaan en enige anorganische zouten op de infectie van Nicotiana glutinosa met tabaksmozaiekvirus. / Liem, A.S.N.

Wageningen : Pudoc, 1963. 46 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - De invloed van auxine, tryptofaan en enige anorganische zouten op de infectie van Nicotiana glutinosa met tabaksmozaiekvirus

AU - Liem, A.S.N.

N1 - WU thesis 345 Proefschrift Wageningen

PY - 1963

Y1 - 1963

KW - tabaksmozaïekvirus

KW - auxinen

KW - nicotiana glutinosa

KW - tryptofaan

KW - anorganische zouten

KW - tobacco mosaic virus

KW - auxins

KW - nicotiana glutinosa

KW - tryptophan

KW - inorganic salts

M3 - internal PhD, WU

PB - Pudoc

CY - Wageningen

ER -