Data mining of in vitro and in vivo dietary intervention datasets

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

The objective of this study was to identify overlap in the response between in vitro (cultured intestinal epithelial cells of pig jejunum, IPEC-j2) and in vivo (pigs, chicken) systems upon (dietary) interventions. We used three different interventions: a high concentration of ZnO, rye, and amoxicillin. In the in vitro system we also challenged the cells by administrating Salmonella after the dietary exposure to trigger an immune reaction. Our approach was to use bioinformatic tools and data mining to investigate to what extent the in vitro system is able to predict the in vivo effects by identification of overlapping genes and biological processes affected by the intervention. Here, we focused mainly on genes, pathways, and biological processes involved in intestinal functionality and immune competence. We only found a minor overlap in genes or processes between both systems. For ZnO the main overlapping process was related to the HIF-1a pathway, whereas for rye we observed many overlapping processes related to cell cycle, and for amoxicillin the reactive oxygen species (ROS) was overlapping. ROS activates CREB activity which may lead to apoptosis. For the in vitro system we observed an up-regulation of key cytokines (IL6 and IL8) in the control samples. In the in vitro system, ZnO and amoxicillin were both able to down-regulate the Salmonella induced inflammatory genes. In the in vivo system ZnO also regulates genes involved in immune related processes (i.e. TGFß, cytokine, and chemokine signaling), whereas the amoxicillin treatment led to higher expression of genes involved in the gut barrier function. In conclusion, although we only observed a minor overlap between the in vitro and in vivo system, this bioinformatics and data mining approach showed that the in vitro system gives an impression of the in vivo system. Potentially, such an approach may yield new angles for selecting compounds for dietary interventions.
Original languageEnglish
Title of host publicationBook of Abstracts of the 66th Annual Meeting of the EAAP
PublisherWageningen Academic Publishers
Pages412-412
Volume21
ISBN (Print)9789086862696
Publication statusPublished - 2015
EventEAAP - 66th Annual Meeting 2015 - Warsaw, Poland
Duration: 31 Aug 20154 Sep 2015

Conference

ConferenceEAAP - 66th Annual Meeting 2015
CountryPoland
CityWarsaw
Period31/08/154/09/15

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