D-Xylose Concentration-Dependent Hydrolase Expression Profiles and the Function of CreA and XlnR in Aspergillus niger

A.R. Mach-Aigner, J. Omony, B. Jovanovic, A.J.B. van Boxtel, L.H. de Graaff

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)

Abstract

Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, d-xylose, is an inducing substance for the coordinate expression of a large number of polysaccharide-degrading enzymes. In this study, the responses of 22 genes to low (1 mM) and high (50 mM) d-xylose concentrations were investigated. These 22 genes encode enzymes that function as xylan backbone-degrading enzymes, accessory enzymes, cellulose-degrading enzymes, or enzymes involved in the pentose catabolic pathway in A. niger. Notably, genes encoding enzymes that have a similar function (e.g., xylan backbone degradation) respond in a similar manner to different concentrations of d-xylose. Although low d-xylose concentrations provoke the greatest change in transcript levels, in particular, for hemicellulase-encoding genes, transcript formation in the presence of high concentrations of d-xylose was also observed. Interestingly, a high d-xylose concentration is favorable for certain groups of genes. Furthermore, the repressing influence of CreA on the transcription and transcript levels of a subset of these genes was observed regardless of whether a low or high concentration of d-xylose was used. Interestingly, the decrease in transcript levels of certain genes on high d-xylose concentrations is not reflected by the transcript level of their activator, XlnR. Regardless of the d-xylose concentration applied and whether CreA was functional, xlnR was constitutively expressed at a low level
Original languageEnglish
Pages (from-to)3145-3155
JournalApplied and Environmental Microbiology
Volume78
Issue number9
DOIs
Publication statusPublished - 2012

Fingerprint

Aspergillus niger
Xylose
Hydrolases
hydrolases
xylose
enzyme
gene
Enzymes
enzymes
Xylans
xylan
Genes
genes
Pentoses
pentoses
polysaccharide
cellulose
Cellulose
Polysaccharides
polysaccharides

Keywords

  • transcriptional activator xlnr
  • jecorina trichoderma-reesei
  • cell-wall polysaccharides
  • time rt-pcr
  • hypocrea-jecorina
  • encoding genes
  • xylanase expression
  • beta-xylosidase
  • enzyme-system
  • l-arabitol

Cite this

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title = "D-Xylose Concentration-Dependent Hydrolase Expression Profiles and the Function of CreA and XlnR in Aspergillus niger",
abstract = "Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, d-xylose, is an inducing substance for the coordinate expression of a large number of polysaccharide-degrading enzymes. In this study, the responses of 22 genes to low (1 mM) and high (50 mM) d-xylose concentrations were investigated. These 22 genes encode enzymes that function as xylan backbone-degrading enzymes, accessory enzymes, cellulose-degrading enzymes, or enzymes involved in the pentose catabolic pathway in A. niger. Notably, genes encoding enzymes that have a similar function (e.g., xylan backbone degradation) respond in a similar manner to different concentrations of d-xylose. Although low d-xylose concentrations provoke the greatest change in transcript levels, in particular, for hemicellulase-encoding genes, transcript formation in the presence of high concentrations of d-xylose was also observed. Interestingly, a high d-xylose concentration is favorable for certain groups of genes. Furthermore, the repressing influence of CreA on the transcription and transcript levels of a subset of these genes was observed regardless of whether a low or high concentration of d-xylose was used. Interestingly, the decrease in transcript levels of certain genes on high d-xylose concentrations is not reflected by the transcript level of their activator, XlnR. Regardless of the d-xylose concentration applied and whether CreA was functional, xlnR was constitutively expressed at a low level",
keywords = "transcriptional activator xlnr, jecorina trichoderma-reesei, cell-wall polysaccharides, time rt-pcr, hypocrea-jecorina, encoding genes, xylanase expression, beta-xylosidase, enzyme-system, l-arabitol",
author = "A.R. Mach-Aigner and J. Omony and B. Jovanovic and {van Boxtel}, A.J.B. and {de Graaff}, L.H.",
year = "2012",
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language = "English",
volume = "78",
pages = "3145--3155",
journal = "Applied and Environmental Microbiology",
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publisher = "American Society for Microbiology",
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}

D-Xylose Concentration-Dependent Hydrolase Expression Profiles and the Function of CreA and XlnR in Aspergillus niger. / Mach-Aigner, A.R.; Omony, J.; Jovanovic, B.; van Boxtel, A.J.B.; de Graaff, L.H.

In: Applied and Environmental Microbiology, Vol. 78, No. 9, 2012, p. 3145-3155.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - D-Xylose Concentration-Dependent Hydrolase Expression Profiles and the Function of CreA and XlnR in Aspergillus niger

AU - Mach-Aigner, A.R.

AU - Omony, J.

AU - Jovanovic, B.

AU - van Boxtel, A.J.B.

AU - de Graaff, L.H.

PY - 2012

Y1 - 2012

N2 - Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, d-xylose, is an inducing substance for the coordinate expression of a large number of polysaccharide-degrading enzymes. In this study, the responses of 22 genes to low (1 mM) and high (50 mM) d-xylose concentrations were investigated. These 22 genes encode enzymes that function as xylan backbone-degrading enzymes, accessory enzymes, cellulose-degrading enzymes, or enzymes involved in the pentose catabolic pathway in A. niger. Notably, genes encoding enzymes that have a similar function (e.g., xylan backbone degradation) respond in a similar manner to different concentrations of d-xylose. Although low d-xylose concentrations provoke the greatest change in transcript levels, in particular, for hemicellulase-encoding genes, transcript formation in the presence of high concentrations of d-xylose was also observed. Interestingly, a high d-xylose concentration is favorable for certain groups of genes. Furthermore, the repressing influence of CreA on the transcription and transcript levels of a subset of these genes was observed regardless of whether a low or high concentration of d-xylose was used. Interestingly, the decrease in transcript levels of certain genes on high d-xylose concentrations is not reflected by the transcript level of their activator, XlnR. Regardless of the d-xylose concentration applied and whether CreA was functional, xlnR was constitutively expressed at a low level

AB - Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, d-xylose, is an inducing substance for the coordinate expression of a large number of polysaccharide-degrading enzymes. In this study, the responses of 22 genes to low (1 mM) and high (50 mM) d-xylose concentrations were investigated. These 22 genes encode enzymes that function as xylan backbone-degrading enzymes, accessory enzymes, cellulose-degrading enzymes, or enzymes involved in the pentose catabolic pathway in A. niger. Notably, genes encoding enzymes that have a similar function (e.g., xylan backbone degradation) respond in a similar manner to different concentrations of d-xylose. Although low d-xylose concentrations provoke the greatest change in transcript levels, in particular, for hemicellulase-encoding genes, transcript formation in the presence of high concentrations of d-xylose was also observed. Interestingly, a high d-xylose concentration is favorable for certain groups of genes. Furthermore, the repressing influence of CreA on the transcription and transcript levels of a subset of these genes was observed regardless of whether a low or high concentration of d-xylose was used. Interestingly, the decrease in transcript levels of certain genes on high d-xylose concentrations is not reflected by the transcript level of their activator, XlnR. Regardless of the d-xylose concentration applied and whether CreA was functional, xlnR was constitutively expressed at a low level

KW - transcriptional activator xlnr

KW - jecorina trichoderma-reesei

KW - cell-wall polysaccharides

KW - time rt-pcr

KW - hypocrea-jecorina

KW - encoding genes

KW - xylanase expression

KW - beta-xylosidase

KW - enzyme-system

KW - l-arabitol

U2 - 10.1128/AEM.07772-11

DO - 10.1128/AEM.07772-11

M3 - Article

VL - 78

SP - 3145

EP - 3155

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 9

ER -