Cytogenetic analysis of quinoa chromosomes using nanoscale imaging and spectroscopy techniques

Zhong Yangquanwei*, Suresh Neethirajan, Chithra Karunakaran

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Here we present a high-resolution chromosomal spectral map derived from synchrotronbased soft X-ray spectromicroscopy applied to quinoa species. The label-free characterization of quinoa metaphase chromosomes shows that it consists of organized substructures of DNAprotein complex. The analysis of spectra of chromosomes using the scanning transmission Xray microscope (STXM) and its superposition of the pattern with the atomic force microscopy (AFM) and scanning electron microscopy (SEM) images proves that it is possible to precisely locate the gene loci and the DNA packaging inside the chromosomes. STXM has been successfully used to distinguish and quantify the DNA and protein components inside the quinoa chromosomes by visualizing the interphase at up to 30-nm spatial resolution. Our study represents the successful attempt of non-intrusive interrogation and integrating imaging techniques of chromosomes using synchrotron STXM and AFM techniques. The methodology developed for 3-D imaging of chromosomes with chemical specificity and temporal resolution will allow the nanoscale imaging tools to emerge from scientific research and development into broad practical applications such as gene loci tools and biomarker libraries.

Original languageEnglish
Article number463
JournalNanoscale Research Letters
Volume8
Issue number1
DOIs
Publication statusPublished - Nov 2013
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Chromosomes
  • Cytogenetics
  • DNA-protein composition
  • Image analysis
  • Soft X-ray spectromicroscopy
  • Synchrotron radiation

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