Cystathionine beta-synthase polymorphisms and hyperhomocysteinaemia: an association study

K.J.A. Lievers, L.A.J. Kluijtmans, S.G. Heil, G.H. Boers, P. Verhoef, M. Den Heijer, F.J. Trijbels, H.J. Blom

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Hyperhomocysteinaemia is generally accepted as an independent and graded risk factor for both arterial occlusive disease and venous thrombosis. The only way of homocysteine degradation is conversion to cysteine in the transsulfuration pathway in which the regulating step is catalysed by cystathionine beta-synthase (CBS). Mild impairment of CBS function could therefore affect homocysteine concentration, in particular after methionine loading, and consequently cardiovascular disease (CVD) risk. We analysed two silent polymorphisms and one short tandem repeat in the CBS gene (ie 699C-->T, 1080C-->T and -5697 (GT) STR) as genetic markers potentially in linkage disequilibrium with a functional polymorphism. We assessed their association with fasting and post-methionine load homocysteine in 190 patients with arterial occlusive disease, and in 381 controls. No differences in CBS genotype frequencies between cases and controls were found, nor was a particular CBS genotype associated with an elevated risk of arterial occlusive disease. Although we did find a high rate of linkage disequilibrium between the two single nucleotide polymorphisms and the GT STR, none of the genotypes defined by the three CBS variants studied showed an association with elevated fasting, post-load or increase upon methionine loading homocysteine concentrations. In conclusion, we did not find any indication that genetic variation in the CBS gene is associated with increased homocysteine concentrations.
Original languageEnglish
Pages (from-to)23-29
JournalEuropean Journal of Human Genetics
Volume11
DOIs
Publication statusPublished - 2003

Keywords

  • coronary-artery disease
  • risk factor
  • total homocysteine
  • vascular-disease
  • mild hyperhomocysteinemia
  • plasma homocysteine
  • heart-disease
  • cbs gene
  • cardiovascular-disease
  • linkage disequilibrium

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