Cyanobacterial flv4-2 operon-encoded proteins optimize light harvesting and charge separation in photosystem II

V. Chukhutsina, L. Bersanini, E. Aro, H. van Amerongen

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Photosystem II (PSII) complexes drive the water splitting reaction necessary to transform sunlight into chemical energy. However, too much light can damage and disrupt PSII. In cyanobacteria, the flv4-2 operon encodes three proteins (Flv2, Flv4 and Sll0218), which safeguard PSII activity under air-level CO2 and in high-light conditions. However, the exact mechanism of action of these proteins has not been clarified yet. We demonstrate that the PSII electron transfer properties are influenced by the flv4-2 operon-encoded proteins. Accelerated secondary charge separation kinetics was observed upon expression/overexpression of the flv4-2 operon. This is likely induced by docking of the Flv2/Flv4 heterodimer in the vicinity of the QB pocket of PSII which, in turn, increases the QB redox potential and consequently stabilizes forward electron transfer. The alternative electron transfer route constituted by Flv2/Flv4 sequesters electrons from QB - guaranteeing the dissipation of excess excitation energy in PSII under stressful conditions. In addition, we demonstrate that in the absence of the flv4-2 operonencoded proteins about 20% of the phycobilisome antenna becomes detached from the reaction centers, thus decreasing light harvesting. Phycobilisome detachment is a consequence of a decreased relative content of PSII dimers, a feature observed in the absence of the Sll0218 protein.
Original languageEnglish
Pages (from-to)747-761
JournalMolecular Plant
Volume8
Issue number5
DOIs
Publication statusPublished - 2015

Keywords

  • sp pcc 6803
  • freeze-fracture particles
  • flavodiiron proteins
  • thermo-luminescence
  • picosecond kinetics
  • thermosynechococcus-elongatus
  • functional-characterization
  • delayed luminescence
  • energy-dissipation
  • state transitions

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