Current Methods for Extraction and Concentration of Enteric Viruses from Fresh Fruit and Vegetables: Towards International Standards

L. Croci, E. Dubois, N. Cook, D. Medici, A.C. Schultz, B. China, S.A. Rutjes, J. Hoorfar, W.H.M. van der Poel

    Research output: Contribution to journalArticleAcademicpeer-review

    46 Citations (Scopus)

    Abstract

    Virus-contaminated soft fruits or vegetables are increasingly identified as causes of foodborne viral illness. Noroviruses and hepatitis A virus are the most common pathogens in viral infections transmitted by these kinds of foods. To improve microbiological detection and monitoring and to increase insights into the contribution of fruits and vegetables to foodborne viral transmission, sensitive, reliable, and standardized methods are needed. More studies on virus detection methods for foods are being published, but validated consensus protocols are not yet available. In this paper, different methodologies are reviewed critically. The use of process controls and internal amplification controls is discussed.
    Original languageEnglish
    Pages (from-to)73-84
    JournalFood Analytical Methods
    Volume1
    Issue number2
    DOIs
    Publication statusPublished - 2008

    Keywords

    • hepatitis-a-virus
    • reverse transcription-pcr
    • sequence-based amplification
    • polymerase-chain-reaction
    • round-structured viruses
    • avian encephalomyelitis virus
    • feline calicivirus
    • rt-pcr
    • frozen raspberries
    • foodborne viruses

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