Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology

Sandra Wiegand, Mareike Jogler, Christian Boedeker, Daniela Pinto, John Vollmers, Elena Rivas-Marín, Timo Kohn, Stijn H. Peeters, Anja Heuer, Patrick Rast, Sonja Oberbeckmann, Boyke Bunk, Olga Jeske, Anke Meyerdierks, Julia E. Storesund, Nicolai Kallscheuer, Sebastian Lücker, Olga M. Lage, Thomas Pohl, Broder J. MerkelPeter Hornburger, Ralph Walter Müller, Franz Brümmer, Matthias Labrenz, Alfred M. Spormann, Huub J.M. Op den Camp, Jörg Overmann, Rudolf Amann, Mike S.M. Jetten, Thorsten Mascher, Marnix H. Medema, Damien P. Devos, Anne Kristin Kaster, Lise Øvreås, Manfred Rohde, Michael Y. Galperin, Christian Jogler*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)


When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that ‘everything is everywhere’. With the discovery of lateral budding in ‘Kolteria novifilia’ and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how ‘microbial dark matter’ can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection.

Original languageEnglish
Pages (from-to)126-140
JournalNature Microbiology
Publication statusPublished - 18 Nov 2019

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    Wiegand, S., Jogler, M., Boedeker, C., Pinto, D., Vollmers, J., Rivas-Marín, E., Kohn, T., Peeters, S. H., Heuer, A., Rast, P., Oberbeckmann, S., Bunk, B., Jeske, O., Meyerdierks, A., Storesund, J. E., Kallscheuer, N., Lücker, S., Lage, O. M., Pohl, T., ... Jogler, C. (2019). Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology. Nature Microbiology, 5, 126-140.