CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD

Shaoyun Dong; Xin Liu; Jianan Han; Han Miao; Diane M. Beckles; Yuling Bai; Xiaoping Liu; Jiantao Guan; Ruizhen Yang; Xingfang Gu; Jiaqiang Sun; Xueyong Yang; Shengping Zhang

doi:10.1093/hr/uhad295

CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD

Shaoyun Dong, Xin Liu, Jianan Han, Han Miao, Diane M. Beckles, Yuling Bai, Xiaoping Liu, Jiantao Guan, Ruizhen Yang, Xingfang Gu, Jiaqiang Sun^*, Xueyong Yang^*, Shengping Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

Powdery mildew (PM) is one of the most destructive diseases that threaten cucumber production globally. Efficient breeding of novel PM-resistant cultivars will require a robust understanding of the molecular mechanisms of cucumber resistance against PM. Using a genome-wide association study, we detected a locus significantly correlated with PM resistance in cucumber stem, pm-s5.1. A 1449-bp insertion in the CsMLO8 coding region at the pm-s5.1 locus resulted in enhanced stem PM resistance. Knockout mutants of CsMLO8 and CsMLO11 generated by CRISPR/Cas9 both showed improved PM resistance in the stem, hypocotyl, and leaves, and the double mutant mlo8mlo11 displayed even stronger resistance. We found that reactive oxygen species (ROS) accumulation was higher in the stem of these mutants. Protein interaction assays suggested that CsMLO8 and CsMLO11 could physically interact with CsRbohD and CsCRK2, respectively. Further, we showed that CsMLO8 and CsCRK2 competitively interact with the C-terminus of CsRbohD to affect CsCRK2-CsRbohD module-mediated ROS production during PM defense. These findings provide new insights into the understanding of CsMLO proteins during PM defense responses.

Original language	English
Article number	uhad295
Journal	Horticulture Research
Volume	11
Issue number	2
DOIs	https://doi.org/10.1093/hr/uhad295
Publication status	Published - 17 Feb 2024

Access to Document

10.1093/hr/uhad295Licence: CC BY

https://edepot.wur.nl/651054Licence: CC BY

Cite this

@article{fdf90ab951684eb1b8ac27c3c762f35a,

title = "CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD",

abstract = "Powdery mildew (PM) is one of the most destructive diseases that threaten cucumber production globally. Efficient breeding of novel PM-resistant cultivars will require a robust understanding of the molecular mechanisms of cucumber resistance against PM. Using a genome-wide association study, we detected a locus significantly correlated with PM resistance in cucumber stem, pm-s5.1. A 1449-bp insertion in the CsMLO8 coding region at the pm-s5.1 locus resulted in enhanced stem PM resistance. Knockout mutants of CsMLO8 and CsMLO11 generated by CRISPR/Cas9 both showed improved PM resistance in the stem, hypocotyl, and leaves, and the double mutant mlo8mlo11 displayed even stronger resistance. We found that reactive oxygen species (ROS) accumulation was higher in the stem of these mutants. Protein interaction assays suggested that CsMLO8 and CsMLO11 could physically interact with CsRbohD and CsCRK2, respectively. Further, we showed that CsMLO8 and CsCRK2 competitively interact with the C-terminus of CsRbohD to affect CsCRK2-CsRbohD module-mediated ROS production during PM defense. These findings provide new insights into the understanding of CsMLO proteins during PM defense responses.",

author = "Shaoyun Dong and Xin Liu and Jianan Han and Han Miao and Beckles, {Diane M.} and Yuling Bai and Xiaoping Liu and Jiantao Guan and Ruizhen Yang and Xingfang Gu and Jiaqiang Sun and Xueyong Yang and Shengping Zhang",

year = "2024",

month = feb,

day = "17",

doi = "10.1093/hr/uhad295",

language = "English",

volume = "11",

journal = "Horticulture Research",

issn = "2052-7276",

publisher = "Nature",

number = "2",

}

TY - JOUR

T1 - CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD

AU - Dong, Shaoyun

AU - Liu, Xin

AU - Han, Jianan

AU - Miao, Han

AU - Beckles, Diane M.

AU - Bai, Yuling

AU - Liu, Xiaoping

AU - Guan, Jiantao

AU - Yang, Ruizhen

AU - Gu, Xingfang

AU - Sun, Jiaqiang

AU - Yang, Xueyong

AU - Zhang, Shengping

PY - 2024/2/17

Y1 - 2024/2/17

N2 - Powdery mildew (PM) is one of the most destructive diseases that threaten cucumber production globally. Efficient breeding of novel PM-resistant cultivars will require a robust understanding of the molecular mechanisms of cucumber resistance against PM. Using a genome-wide association study, we detected a locus significantly correlated with PM resistance in cucumber stem, pm-s5.1. A 1449-bp insertion in the CsMLO8 coding region at the pm-s5.1 locus resulted in enhanced stem PM resistance. Knockout mutants of CsMLO8 and CsMLO11 generated by CRISPR/Cas9 both showed improved PM resistance in the stem, hypocotyl, and leaves, and the double mutant mlo8mlo11 displayed even stronger resistance. We found that reactive oxygen species (ROS) accumulation was higher in the stem of these mutants. Protein interaction assays suggested that CsMLO8 and CsMLO11 could physically interact with CsRbohD and CsCRK2, respectively. Further, we showed that CsMLO8 and CsCRK2 competitively interact with the C-terminus of CsRbohD to affect CsCRK2-CsRbohD module-mediated ROS production during PM defense. These findings provide new insights into the understanding of CsMLO proteins during PM defense responses.

AB - Powdery mildew (PM) is one of the most destructive diseases that threaten cucumber production globally. Efficient breeding of novel PM-resistant cultivars will require a robust understanding of the molecular mechanisms of cucumber resistance against PM. Using a genome-wide association study, we detected a locus significantly correlated with PM resistance in cucumber stem, pm-s5.1. A 1449-bp insertion in the CsMLO8 coding region at the pm-s5.1 locus resulted in enhanced stem PM resistance. Knockout mutants of CsMLO8 and CsMLO11 generated by CRISPR/Cas9 both showed improved PM resistance in the stem, hypocotyl, and leaves, and the double mutant mlo8mlo11 displayed even stronger resistance. We found that reactive oxygen species (ROS) accumulation was higher in the stem of these mutants. Protein interaction assays suggested that CsMLO8 and CsMLO11 could physically interact with CsRbohD and CsCRK2, respectively. Further, we showed that CsMLO8 and CsCRK2 competitively interact with the C-terminus of CsRbohD to affect CsCRK2-CsRbohD module-mediated ROS production during PM defense. These findings provide new insights into the understanding of CsMLO proteins during PM defense responses.

U2 - 10.1093/hr/uhad295

DO - 10.1093/hr/uhad295

M3 - Article

SN - 2052-7276

VL - 11

JO - Horticulture Research

JF - Horticulture Research

IS - 2

M1 - uhad295

ER -

CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD

Abstract

Access to Document

Fingerprint

Cite this