Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P21, with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 Å, ß = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.3 Å3 Da1 and the solvent content is 47%.
|Journal||Acta Crystallographica Section F. Structural Biology and Crystallization Communications|
|Publication status||Published - 2007|
Sun, L., Levisson, M., Hendriks, S. N. A., Akveld, T., Kengen, S. W. M., Dijkstra, B. W., & van der Oost, J. (2007). Crystallization and preliminary crystallographic analysis of an esterese with a novel domein from the hyperthermophile Thermotoga maritima. Acta Crystallographica Section F. Structural Biology and Crystallization Communications, 63(9), 777-779. https://doi.org/10.1107/S174430910703953X