Crystallization and preliminary crystallographic analysis of an esterese with a novel domein from the hyperthermophile Thermotoga maritima

L. Sun, M. Levisson, S.N.A. Hendriks, T. Akveld, S.W.M. Kengen, B.W. Dijkstra, J. van der Oost

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P21, with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 Å, ß = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.3 Å3 Da1 and the solvent content is 47%.
Original languageEnglish
Pages (from-to)777-779
JournalActa Crystallographica Section F. Structural Biology and Crystallization Communications
Volume63
Issue number9
DOIs
Publication statusPublished - 2007

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