Cruciferous Vegetable Consumption, Glutathione-S-Transferase Polymorphisms, and Colorectal Adenoma Risk in a Dutch Case-Control Study

M.J. Tijhuis, P.A. Wark, J.M.M.J.G. Aarts, M.H.P.W. Visker, F.M. Nagengast, F.J. Kok, E. Kampman

Research output: Contribution to journalAbstractAcademic


Glutathione S-transferases (GSTs) metabolize cancer-protective compounds from cruciferous vegetables and may differentially do so depending on genetic variation. The possible interplay among cruciferous vegetable consumption; functional genetic variations in GST M1, T1, P1, and A1; and colorectal adenomas was investigated in a Dutch case-control study. The study included 746 subjects with at least 1 histologically confirmed colorectal adenomatous polyp ever in their lives and 698 controls without any type of colorectal polyp in their medical history and at index colonoscopy. Dietary habits were assessed by the Dutch version of the European Investigation into Cancer food frequency questionnaire. Other lifestyle factors, such as smoking habits, were determined by a general questionnaire. Functional polymorphisms in 4 GST isoforms were assessed in DNA isolated from whole blood: the GSTM1 and GSTT1 deletion polymorphisms and single-nucleotide polymorphisms in GSTP1 (A313G) and in GSTA1 (C-69T, part of a functional haplotype). High- and low-consumption groups were formed based on a median split in the control group. Consumption of cruciferous vegetables was slightly positively associated with colorectal adenomas, odds ratio (OR) 1.15 (95% CI: 0.92, 1.44). When genotype was taken into account, a positive association with high brassica intake was only apparent in individuals with the low-activity GSTP1 allele (GG-genotype, adjusted OR 1.94; 95% CI: 1.02, 3.69), and this interaction was more pronounced in men, with higher age and with higher red- and processed-meat consumption. There may be a modifying role for the GSTA1 variant as well: the OR with higher intake compared with lower intake was 1.59 (95% CI: 0.94, 2.69) for individuals homozygous for the low expression variant (TT genotype). This was most apparent with younger age and higher meat intake. The GSTM1 and GSTT1 genotypes did not modify the association between cruciferous vegetable intake and colorectal adenomas
Original languageEnglish
Pages (from-to)3044S-3044S
JournalThe Journal of Nutrition
Issue number12
Publication statusPublished - Dec 2005


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