Cross-Species Comparison of Genes Related to Nutrient Sensing Mechanisms Expressed along the Intestine

N. van der Wielen, M. van Avesaat, N.J.W. de Wit, J. Vogels, F. Troost, A. Masclee, S.J. Koopmans, J. van der Meulen, M.V. Boekschoten, M.R. Müller, H.F.J. Hendriks, R.F. Witkamp, J. Meijerink

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Abstract

Introduction Intestinal chemosensory receptors and transporters are able to detect food-derived molecules and are involved in the modulation of gut hormone release. Gut hormones play an important role in the regulation of food intake and the control of gastrointestinal functioning. This mechanism is often referred to as “nutrient sensing”. Knowledge of the distribution of chemosensors along the intestinal tract is important to gain insight in nutrient detection and sensing, both pivotal processes for the regulation of food intake. However, most knowledge is derived from rodents, whereas studies in man and pig are limited, and cross-species comparisons are lacking. Aim To characterize and compare intestinal expression patterns of genes related to nutrient sensing in mice, pigs and humans. Methods Mucosal biopsy samples taken at six locations in human intestine (n = 40) were analyzed by qPCR. Intestinal scrapings from 14 locations in pigs (n = 6) and from 10 locations in mice (n = 4) were analyzed by qPCR and microarray, respectively. The gene expression of glucagon, cholecystokinin, peptide YY, glucagon-like peptide-1 receptor, taste receptor T1R3, sodium/glucose cotransporter, peptide transporter-1, GPR120, taste receptor T1R1, GPR119 and GPR93 was investigated. Partial least squares (PLS) modeling was used to compare the intestinal expression pattern between the three species. Results and conclusion The studied genes were found to display specific expression patterns along the intestinal tract. PLS analysis showed a high similarity between human, pig and mouse in the expression of genes related to nutrient sensing in the distal ileum, and between human and pig in the colon. The gene expression pattern was most deviating between the species in the proximal intestine. Our results give new insights in interspecies similarities and provide new leads for translational research and models aiming to modulate food intake processes in man.
Original languageEnglish
Article numbere107531
Number of pages9
JournalPLoS ONE
Volume9
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

Nutrients
Intestines
intestines
Swine
Genes
Food
nutrients
Appetite Regulation
Gene Expression
swine
Gene expression
food intake
Least-Squares Analysis
genes
Sodium-Glucose Transport Proteins
Hormones
Peptide YY
gene expression
least squares
Biopsy

Keywords

  • glucagon-like peptide-1
  • h+/peptide transporter pept1
  • glucose cotransporter sglt1
  • dietary-regulation
  • molecular-mechanisms
  • receptor expression
  • sucrase-isomaltase
  • hormone-secretion
  • taste receptors
  • induced obesity

Cite this

@article{b720e4828b6e45dfa394d332a8bfe9cf,
title = "Cross-Species Comparison of Genes Related to Nutrient Sensing Mechanisms Expressed along the Intestine",
abstract = "Introduction Intestinal chemosensory receptors and transporters are able to detect food-derived molecules and are involved in the modulation of gut hormone release. Gut hormones play an important role in the regulation of food intake and the control of gastrointestinal functioning. This mechanism is often referred to as “nutrient sensing”. Knowledge of the distribution of chemosensors along the intestinal tract is important to gain insight in nutrient detection and sensing, both pivotal processes for the regulation of food intake. However, most knowledge is derived from rodents, whereas studies in man and pig are limited, and cross-species comparisons are lacking. Aim To characterize and compare intestinal expression patterns of genes related to nutrient sensing in mice, pigs and humans. Methods Mucosal biopsy samples taken at six locations in human intestine (n = 40) were analyzed by qPCR. Intestinal scrapings from 14 locations in pigs (n = 6) and from 10 locations in mice (n = 4) were analyzed by qPCR and microarray, respectively. The gene expression of glucagon, cholecystokinin, peptide YY, glucagon-like peptide-1 receptor, taste receptor T1R3, sodium/glucose cotransporter, peptide transporter-1, GPR120, taste receptor T1R1, GPR119 and GPR93 was investigated. Partial least squares (PLS) modeling was used to compare the intestinal expression pattern between the three species. Results and conclusion The studied genes were found to display specific expression patterns along the intestinal tract. PLS analysis showed a high similarity between human, pig and mouse in the expression of genes related to nutrient sensing in the distal ileum, and between human and pig in the colon. The gene expression pattern was most deviating between the species in the proximal intestine. Our results give new insights in interspecies similarities and provide new leads for translational research and models aiming to modulate food intake processes in man.",
keywords = "glucagon-like peptide-1, h+/peptide transporter pept1, glucose cotransporter sglt1, dietary-regulation, molecular-mechanisms, receptor expression, sucrase-isomaltase, hormone-secretion, taste receptors, induced obesity",
author = "{van der Wielen}, N. and {van Avesaat}, M. and {de Wit}, N.J.W. and J. Vogels and F. Troost and A. Masclee and S.J. Koopmans and {van der Meulen}, J. and M.V. Boekschoten and M.R. M{\"u}ller and H.F.J. Hendriks and R.F. Witkamp and J. Meijerink",
year = "2014",
doi = "10.1371/journal.pone.0107531",
language = "English",
volume = "9",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

Cross-Species Comparison of Genes Related to Nutrient Sensing Mechanisms Expressed along the Intestine. / van der Wielen, N.; van Avesaat, M.; de Wit, N.J.W.; Vogels, J.; Troost, F.; Masclee, A.; Koopmans, S.J.; van der Meulen, J.; Boekschoten, M.V.; Müller, M.R.; Hendriks, H.F.J.; Witkamp, R.F.; Meijerink, J.

In: PLoS ONE, Vol. 9, No. 9, e107531, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cross-Species Comparison of Genes Related to Nutrient Sensing Mechanisms Expressed along the Intestine

AU - van der Wielen, N.

AU - van Avesaat, M.

AU - de Wit, N.J.W.

AU - Vogels, J.

AU - Troost, F.

AU - Masclee, A.

AU - Koopmans, S.J.

AU - van der Meulen, J.

AU - Boekschoten, M.V.

AU - Müller, M.R.

AU - Hendriks, H.F.J.

AU - Witkamp, R.F.

AU - Meijerink, J.

PY - 2014

Y1 - 2014

N2 - Introduction Intestinal chemosensory receptors and transporters are able to detect food-derived molecules and are involved in the modulation of gut hormone release. Gut hormones play an important role in the regulation of food intake and the control of gastrointestinal functioning. This mechanism is often referred to as “nutrient sensing”. Knowledge of the distribution of chemosensors along the intestinal tract is important to gain insight in nutrient detection and sensing, both pivotal processes for the regulation of food intake. However, most knowledge is derived from rodents, whereas studies in man and pig are limited, and cross-species comparisons are lacking. Aim To characterize and compare intestinal expression patterns of genes related to nutrient sensing in mice, pigs and humans. Methods Mucosal biopsy samples taken at six locations in human intestine (n = 40) were analyzed by qPCR. Intestinal scrapings from 14 locations in pigs (n = 6) and from 10 locations in mice (n = 4) were analyzed by qPCR and microarray, respectively. The gene expression of glucagon, cholecystokinin, peptide YY, glucagon-like peptide-1 receptor, taste receptor T1R3, sodium/glucose cotransporter, peptide transporter-1, GPR120, taste receptor T1R1, GPR119 and GPR93 was investigated. Partial least squares (PLS) modeling was used to compare the intestinal expression pattern between the three species. Results and conclusion The studied genes were found to display specific expression patterns along the intestinal tract. PLS analysis showed a high similarity between human, pig and mouse in the expression of genes related to nutrient sensing in the distal ileum, and between human and pig in the colon. The gene expression pattern was most deviating between the species in the proximal intestine. Our results give new insights in interspecies similarities and provide new leads for translational research and models aiming to modulate food intake processes in man.

AB - Introduction Intestinal chemosensory receptors and transporters are able to detect food-derived molecules and are involved in the modulation of gut hormone release. Gut hormones play an important role in the regulation of food intake and the control of gastrointestinal functioning. This mechanism is often referred to as “nutrient sensing”. Knowledge of the distribution of chemosensors along the intestinal tract is important to gain insight in nutrient detection and sensing, both pivotal processes for the regulation of food intake. However, most knowledge is derived from rodents, whereas studies in man and pig are limited, and cross-species comparisons are lacking. Aim To characterize and compare intestinal expression patterns of genes related to nutrient sensing in mice, pigs and humans. Methods Mucosal biopsy samples taken at six locations in human intestine (n = 40) were analyzed by qPCR. Intestinal scrapings from 14 locations in pigs (n = 6) and from 10 locations in mice (n = 4) were analyzed by qPCR and microarray, respectively. The gene expression of glucagon, cholecystokinin, peptide YY, glucagon-like peptide-1 receptor, taste receptor T1R3, sodium/glucose cotransporter, peptide transporter-1, GPR120, taste receptor T1R1, GPR119 and GPR93 was investigated. Partial least squares (PLS) modeling was used to compare the intestinal expression pattern between the three species. Results and conclusion The studied genes were found to display specific expression patterns along the intestinal tract. PLS analysis showed a high similarity between human, pig and mouse in the expression of genes related to nutrient sensing in the distal ileum, and between human and pig in the colon. The gene expression pattern was most deviating between the species in the proximal intestine. Our results give new insights in interspecies similarities and provide new leads for translational research and models aiming to modulate food intake processes in man.

KW - glucagon-like peptide-1

KW - h+/peptide transporter pept1

KW - glucose cotransporter sglt1

KW - dietary-regulation

KW - molecular-mechanisms

KW - receptor expression

KW - sucrase-isomaltase

KW - hormone-secretion

KW - taste receptors

KW - induced obesity

U2 - 10.1371/journal.pone.0107531

DO - 10.1371/journal.pone.0107531

M3 - Article

VL - 9

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

M1 - e107531

ER -