Cross-sectional study on prevalence and molecular characteristics of plasmid mediated ESBL/AmpC-producing Escherichia coli isolated from veal calves at slaughter

J. Hordijk, J.A. Wagenaar, A. Kant, A. van Essen-Zandbergen, C.M. Dierikx, K. Veldman, B. Wit, D.J. Mevius

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26 Citations (Scopus)

Abstract

Objectives - The presence of ESBL/AmpC-producing E. coli in cattle has been reported previously, however information on veal calves is limited. This study describes the prevalence and molecular characteristics of E. coli with non-wild type susceptibility to cefotaxime in veal calves at slaughter. Methods - Faecal samples from 100 herds, 10 individual animals per herd, were screened for E. coli with non-wild type susceptibility for cefotaxime. Molecular characterization of ESBL/AmpC genes and plasmids was performed on one isolate per herd by microarray, PCR and sequence analysis. Results - 66% of the herds were positive for E. coli with non-wild type susceptibility for cefotaxime. Within-herd prevalence varied from zero to 90%. 83% of E. coli producing ESBL/AmpC carried blaCTX-M genes, of which blaCTX-M-1, blaCTX-M-14 and blaCTX-M-15 were most prevalent. The dominant plasmids were IncI1 and IncF-type plasmids. Conclusions - A relatively high prevalence of various blaCTX-M producing E. coli was found in veal calves at slaughter. The genes were mainly located on IncI1 and IncF plasmids.
Original languageEnglish
Article numbere65681
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 2013

Keywords

  • spectrum-beta-lactamase
  • food-producing animals
  • extended-spectrum
  • ctx-m
  • salmonella-enterica
  • fecal carriage
  • antimicrobial resistance
  • host-range
  • cattle
  • enterobacteriaceae

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