Cross-sectional study on prevalence and molecular characteristics of plasmid mediated ESBL/AmpC-producing Escherichia coli isolated from veal calves at slaughter

J. Hordijk, J.A. Wagenaar, A. Kant, A. van Essen-Zandbergen, C.M. Dierikx, K. Veldman, B. Wit, D.J. Mevius

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25 Citations (Scopus)

Abstract

Objectives - The presence of ESBL/AmpC-producing E. coli in cattle has been reported previously, however information on veal calves is limited. This study describes the prevalence and molecular characteristics of E. coli with non-wild type susceptibility to cefotaxime in veal calves at slaughter. Methods - Faecal samples from 100 herds, 10 individual animals per herd, were screened for E. coli with non-wild type susceptibility for cefotaxime. Molecular characterization of ESBL/AmpC genes and plasmids was performed on one isolate per herd by microarray, PCR and sequence analysis. Results - 66% of the herds were positive for E. coli with non-wild type susceptibility for cefotaxime. Within-herd prevalence varied from zero to 90%. 83% of E. coli producing ESBL/AmpC carried blaCTX-M genes, of which blaCTX-M-1, blaCTX-M-14 and blaCTX-M-15 were most prevalent. The dominant plasmids were IncI1 and IncF-type plasmids. Conclusions - A relatively high prevalence of various blaCTX-M producing E. coli was found in veal calves at slaughter. The genes were mainly located on IncI1 and IncF plasmids.
Original languageEnglish
Article numbere65681
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 2013

Fingerprint

veal calves
cross-sectional studies
Escherichia coli
plasmids
slaughter
Plasmids
Cross-Sectional Studies
cefotaxime
herds
Cefotaxime
Genes
genes
Microarray Analysis
Microarrays
Sequence Analysis
Animals
sequence analysis
Polymerase Chain Reaction
cattle
animals

Keywords

  • spectrum-beta-lactamase
  • food-producing animals
  • extended-spectrum
  • ctx-m
  • salmonella-enterica
  • fecal carriage
  • antimicrobial resistance
  • host-range
  • cattle
  • enterobacteriaceae

Cite this

@article{e15df332e1a94f4ea7e0e1d7eac0cd48,
title = "Cross-sectional study on prevalence and molecular characteristics of plasmid mediated ESBL/AmpC-producing Escherichia coli isolated from veal calves at slaughter",
abstract = "Objectives - The presence of ESBL/AmpC-producing E. coli in cattle has been reported previously, however information on veal calves is limited. This study describes the prevalence and molecular characteristics of E. coli with non-wild type susceptibility to cefotaxime in veal calves at slaughter. Methods - Faecal samples from 100 herds, 10 individual animals per herd, were screened for E. coli with non-wild type susceptibility for cefotaxime. Molecular characterization of ESBL/AmpC genes and plasmids was performed on one isolate per herd by microarray, PCR and sequence analysis. Results - 66{\%} of the herds were positive for E. coli with non-wild type susceptibility for cefotaxime. Within-herd prevalence varied from zero to 90{\%}. 83{\%} of E. coli producing ESBL/AmpC carried blaCTX-M genes, of which blaCTX-M-1, blaCTX-M-14 and blaCTX-M-15 were most prevalent. The dominant plasmids were IncI1 and IncF-type plasmids. Conclusions - A relatively high prevalence of various blaCTX-M producing E. coli was found in veal calves at slaughter. The genes were mainly located on IncI1 and IncF plasmids.",
keywords = "spectrum-beta-lactamase, food-producing animals, extended-spectrum, ctx-m, salmonella-enterica, fecal carriage, antimicrobial resistance, host-range, cattle, enterobacteriaceae",
author = "J. Hordijk and J.A. Wagenaar and A. Kant and {van Essen-Zandbergen}, A. and C.M. Dierikx and K. Veldman and B. Wit and D.J. Mevius",
year = "2013",
doi = "10.1371/journal.pone.0065681",
language = "English",
volume = "8",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

Cross-sectional study on prevalence and molecular characteristics of plasmid mediated ESBL/AmpC-producing Escherichia coli isolated from veal calves at slaughter. / Hordijk, J.; Wagenaar, J.A.; Kant, A.; van Essen-Zandbergen, A.; Dierikx, C.M.; Veldman, K.; Wit, B.; Mevius, D.J.

In: PLoS ONE, Vol. 8, No. 5, e65681, 2013.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cross-sectional study on prevalence and molecular characteristics of plasmid mediated ESBL/AmpC-producing Escherichia coli isolated from veal calves at slaughter

AU - Hordijk, J.

AU - Wagenaar, J.A.

AU - Kant, A.

AU - van Essen-Zandbergen, A.

AU - Dierikx, C.M.

AU - Veldman, K.

AU - Wit, B.

AU - Mevius, D.J.

PY - 2013

Y1 - 2013

N2 - Objectives - The presence of ESBL/AmpC-producing E. coli in cattle has been reported previously, however information on veal calves is limited. This study describes the prevalence and molecular characteristics of E. coli with non-wild type susceptibility to cefotaxime in veal calves at slaughter. Methods - Faecal samples from 100 herds, 10 individual animals per herd, were screened for E. coli with non-wild type susceptibility for cefotaxime. Molecular characterization of ESBL/AmpC genes and plasmids was performed on one isolate per herd by microarray, PCR and sequence analysis. Results - 66% of the herds were positive for E. coli with non-wild type susceptibility for cefotaxime. Within-herd prevalence varied from zero to 90%. 83% of E. coli producing ESBL/AmpC carried blaCTX-M genes, of which blaCTX-M-1, blaCTX-M-14 and blaCTX-M-15 were most prevalent. The dominant plasmids were IncI1 and IncF-type plasmids. Conclusions - A relatively high prevalence of various blaCTX-M producing E. coli was found in veal calves at slaughter. The genes were mainly located on IncI1 and IncF plasmids.

AB - Objectives - The presence of ESBL/AmpC-producing E. coli in cattle has been reported previously, however information on veal calves is limited. This study describes the prevalence and molecular characteristics of E. coli with non-wild type susceptibility to cefotaxime in veal calves at slaughter. Methods - Faecal samples from 100 herds, 10 individual animals per herd, were screened for E. coli with non-wild type susceptibility for cefotaxime. Molecular characterization of ESBL/AmpC genes and plasmids was performed on one isolate per herd by microarray, PCR and sequence analysis. Results - 66% of the herds were positive for E. coli with non-wild type susceptibility for cefotaxime. Within-herd prevalence varied from zero to 90%. 83% of E. coli producing ESBL/AmpC carried blaCTX-M genes, of which blaCTX-M-1, blaCTX-M-14 and blaCTX-M-15 were most prevalent. The dominant plasmids were IncI1 and IncF-type plasmids. Conclusions - A relatively high prevalence of various blaCTX-M producing E. coli was found in veal calves at slaughter. The genes were mainly located on IncI1 and IncF plasmids.

KW - spectrum-beta-lactamase

KW - food-producing animals

KW - extended-spectrum

KW - ctx-m

KW - salmonella-enterica

KW - fecal carriage

KW - antimicrobial resistance

KW - host-range

KW - cattle

KW - enterobacteriaceae

U2 - 10.1371/journal.pone.0065681

DO - 10.1371/journal.pone.0065681

M3 - Article

VL - 8

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 5

M1 - e65681

ER -