Critical Steps in DAPI and FISH Imaging of Chromosome Spread Preparations

Hans de Jong*, José van de Belt, Paul Fransz

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


The final step in a long period of chromosome slide experiments is the publication of DAPI and multicolor fluorescence images. Quite often the result of published artwork is disappointing due to insufficient knowledge of image processing and presentation. In this chapter we describe some errors of fluorescence photomicrographs and how to avoid them. We include suggestions of processing chromosome images with simple examples of image processing in Photoshop® or the like, without the need of complex knowledge of the software programs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsT. Heitkam, S. Garcia
PublisherHumana Press
Number of pages10
ISBN (Electronic)9781071632260
ISBN (Print)9781071632284
Publication statusPublished - 20 Jun 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • DAPI
  • FISH
  • Fluorescence microscopy
  • Image processing
  • Imaging


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