CRISPR type V-U1 system from Mycobacterium mucogenicum and uses thereof

John Van Der Oost (Inventor), Prarthana Mohanraju (Inventor), Wen Ying Wu (Inventor), Sjoerd Creutzburg (Inventor)

Research output: PatentOther research output


The type V-U1 system from the bacterium Mycobacterium mucogenicum CCH10-A2 (Mmu) has a nuclease which binds dsDNA but it does not cleave it. Additionally, after dsDNA binding by the nuclease an RuvC-dependent interference of nascent transcript (mRNA) takes place and this mechanism has not been described before for any CRISPR system. CRISPR based gene manipulation can therefore use CRISPR endonucleases from the Type V-U1 system from Mycobacterium mucogenicum, including variant and modified endonucleases, so as to provide for methods of expression control and gene editing in cells of any living organism or of any nucleic acid in vitro.

Original languageEnglish
Patent numberWO2021001534
Priority date3/07/19
Publication statusPublished - 7 Jan 2021

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