Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice

Jeroen Kortekaas, Rianka P.M. Vloet, Alexander J. Mcauley, Xiaoli Shen, Berend Jan Bosch, Laura De Vries, Rob J.M. Moormann, Dennis A. Bente

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Crimean-Congo hemorrhagic fever virus is a tick-borne bunyavirus of the Nairovirus genus that causes hemorrhagic fever in humans with high case fatality. Here, we report the development of subunit vaccines and their efficacy in signal transducer and activator of transcription 1 (STAT1) knockout mice. Ectodomains of the structural glycoproteins Gn and Gc were produced using a Drosophila insect cell-based expression system. A single vaccination of STAT129 mice with adjuvanted Gn or Gc ectodomains induced neutralizing antibody responses, which were boosted by a second vaccination. Despite these antibody responses, mice were not protected from a CCHFV challenge infection. These results suggest that neutralizing antibodies against CCHFV do not correlate with protection of STAT1 knockout mice.

LanguageEnglish
Pages759-764
JournalVector-Borne and Zoonotic Diseases
Volume15
Issue number12
DOIs
Publication statusPublished - 1 Dec 2015

Fingerprint

Crimean-Congo hemorrhagic fever virus
STAT1 Transcription Factor
Subunit Vaccines
Neutralizing Antibodies
Knockout Mice
Antibody Formation
Nairovirus
Vaccination
Orthobunyavirus
Ticks
Drosophila
Insects
Fever
Infection

Keywords

  • Bunyavirus
  • Crimean-Congo hemorrhagic fever virus
  • Gc
  • Gn
  • Nairovirus
  • Neutralizing antibodies
  • STAT1
  • STAT129
  • Vaccine

Cite this

Kortekaas, Jeroen ; Vloet, Rianka P.M. ; Mcauley, Alexander J. ; Shen, Xiaoli ; Bosch, Berend Jan ; De Vries, Laura ; Moormann, Rob J.M. ; Bente, Dennis A. / Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice. In: Vector-Borne and Zoonotic Diseases. 2015 ; Vol. 15, No. 12. pp. 759-764.
@article{c0400e55220144a487f2ab645f22f64b,
title = "Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice",
abstract = "Crimean-Congo hemorrhagic fever virus is a tick-borne bunyavirus of the Nairovirus genus that causes hemorrhagic fever in humans with high case fatality. Here, we report the development of subunit vaccines and their efficacy in signal transducer and activator of transcription 1 (STAT1) knockout mice. Ectodomains of the structural glycoproteins Gn and Gc were produced using a Drosophila insect cell-based expression system. A single vaccination of STAT129 mice with adjuvanted Gn or Gc ectodomains induced neutralizing antibody responses, which were boosted by a second vaccination. Despite these antibody responses, mice were not protected from a CCHFV challenge infection. These results suggest that neutralizing antibodies against CCHFV do not correlate with protection of STAT1 knockout mice.",
keywords = "Bunyavirus, Crimean-Congo hemorrhagic fever virus, Gc, Gn, Nairovirus, Neutralizing antibodies, STAT1, STAT129, Vaccine",
author = "Jeroen Kortekaas and Vloet, {Rianka P.M.} and Mcauley, {Alexander J.} and Xiaoli Shen and Bosch, {Berend Jan} and {De Vries}, Laura and Moormann, {Rob J.M.} and Bente, {Dennis A.}",
year = "2015",
month = "12",
day = "1",
doi = "10.1089/vbz.2015.1855",
language = "English",
volume = "15",
pages = "759--764",
journal = "Vector-Borne and Zoonotic Diseases",
issn = "1530-3667",
publisher = "Mary Ann Liebert",
number = "12",

}

Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice. / Kortekaas, Jeroen; Vloet, Rianka P.M.; Mcauley, Alexander J.; Shen, Xiaoli; Bosch, Berend Jan; De Vries, Laura; Moormann, Rob J.M.; Bente, Dennis A.

In: Vector-Borne and Zoonotic Diseases, Vol. 15, No. 12, 01.12.2015, p. 759-764.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice

AU - Kortekaas, Jeroen

AU - Vloet, Rianka P.M.

AU - Mcauley, Alexander J.

AU - Shen, Xiaoli

AU - Bosch, Berend Jan

AU - De Vries, Laura

AU - Moormann, Rob J.M.

AU - Bente, Dennis A.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Crimean-Congo hemorrhagic fever virus is a tick-borne bunyavirus of the Nairovirus genus that causes hemorrhagic fever in humans with high case fatality. Here, we report the development of subunit vaccines and their efficacy in signal transducer and activator of transcription 1 (STAT1) knockout mice. Ectodomains of the structural glycoproteins Gn and Gc were produced using a Drosophila insect cell-based expression system. A single vaccination of STAT129 mice with adjuvanted Gn or Gc ectodomains induced neutralizing antibody responses, which were boosted by a second vaccination. Despite these antibody responses, mice were not protected from a CCHFV challenge infection. These results suggest that neutralizing antibodies against CCHFV do not correlate with protection of STAT1 knockout mice.

AB - Crimean-Congo hemorrhagic fever virus is a tick-borne bunyavirus of the Nairovirus genus that causes hemorrhagic fever in humans with high case fatality. Here, we report the development of subunit vaccines and their efficacy in signal transducer and activator of transcription 1 (STAT1) knockout mice. Ectodomains of the structural glycoproteins Gn and Gc were produced using a Drosophila insect cell-based expression system. A single vaccination of STAT129 mice with adjuvanted Gn or Gc ectodomains induced neutralizing antibody responses, which were boosted by a second vaccination. Despite these antibody responses, mice were not protected from a CCHFV challenge infection. These results suggest that neutralizing antibodies against CCHFV do not correlate with protection of STAT1 knockout mice.

KW - Bunyavirus

KW - Crimean-Congo hemorrhagic fever virus

KW - Gc

KW - Gn

KW - Nairovirus

KW - Neutralizing antibodies

KW - STAT1

KW - STAT129

KW - Vaccine

U2 - 10.1089/vbz.2015.1855

DO - 10.1089/vbz.2015.1855

M3 - Article

VL - 15

SP - 759

EP - 764

JO - Vector-Borne and Zoonotic Diseases

T2 - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

SN - 1530-3667

IS - 12

ER -