Creation of a Nonspreading Rift Valley Fever Virus

J.A. Kortekaas, N.D. Oreshkova, V. Cobos-Jeménez, R.P.M. Vloet, C. Potgieter, R.J.M. Moormann

Research output: Contribution to journalArticleAcademicpeer-review

57 Citations (Scopus)

Abstract

Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic bunyavirus of the genus Phlebovirus and a serious human and veterinary pathogen. RVFV contains a three-segmented RNA genome, which is comprised of the large (L), medium (M), and small (S) segments. The proteins that are essential for genome replication are encoded by the L and S segments, whereas the structural glycoproteins are encoded by the M segment. We have produced BHK replicon cell lines (BHK-Rep) that maintain replicating L and S genome segments. Transfection of BHK-Rep cells with a plasmid encoding the structural glycoproteins results in the efficient production of RVFV replicon particles (RRPs). To facilitate monitoring of infection, the NSs gene was replaced with an enhanced green fluorescent protein gene. RRPs are infectious for both mammalian and insect cells but are incapable of autonomous spreading, rendering their application outside biosafety containment completely safe. We demonstrate that a single intramuscular vaccination with RRPs protects mice from a lethal dose of RVFV and show that RRPs can be used for rapid virus neutralization tests that do not require biocontainment facilities. The methods reported here will greatly facilitate vaccine and drug development as well as fundamental studies on RVFV biology. Moreover, it may be possible to develop similar systems for other members of the bunyavirus family as well.
Original languageEnglish
Pages (from-to)12622-12630
JournalJournal of Virology
Volume85
Issue number23
DOIs
Publication statusPublished - 2011

Keywords

  • north-american mosquitos
  • mammalian-cells
  • rna-polymerase
  • expression
  • rescue
  • vaccine
  • protein
  • particles
  • cdna
  • gene

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