TY - JOUR
T1 - Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
AU - Zetsche, Bernd
AU - Gootenberg, Jonathan S.
AU - Abudayyeh, Omar O.
AU - Slaymaker, Ian M.
AU - Makarova, Kira S.
AU - Essletzbichler, Patrick
AU - Volz, Sara E.
AU - Joung, Julia
AU - van der Oost, John
AU - Regev, Aviv
AU - Koonin, Eugene V.
AU - Zhang, Feng
PY - 2015
Y1 - 2015
N2 - The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here, we report characterization of Cpf1, a putative class 2 CRISPR effector. We demonstrate that Cpf1 mediates robust DNA interference with features distinct from Cas9. Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif. Moreover, Cpf1 cleaves DNA via a staggered DNA double-stranded break. Out of 16 Cpf1-family proteins, we identified two candidate enzymes from Acidaminococcus and Lachnospiraceae, with efficient genome-editing activity in human cells. Identifying this mechanism of interference broadens our understanding of CRISPR-Cas systems and advances their genome editing applications.
AB - The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here, we report characterization of Cpf1, a putative class 2 CRISPR effector. We demonstrate that Cpf1 mediates robust DNA interference with features distinct from Cas9. Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif. Moreover, Cpf1 cleaves DNA via a staggered DNA double-stranded break. Out of 16 Cpf1-family proteins, we identified two candidate enzymes from Acidaminococcus and Lachnospiraceae, with efficient genome-editing activity in human cells. Identifying this mechanism of interference broadens our understanding of CRISPR-Cas systems and advances their genome editing applications.
U2 - 10.1016/j.cell.2015.09.038
DO - 10.1016/j.cell.2015.09.038
M3 - Article
SN - 0092-8674
VL - 163
SP - 759
EP - 771
JO - Cell
JF - Cell
IS - 3
ER -