Covalent Microcontact Printing of Proteins for Cell Patterning

D.I. Rozkiewicz, Y. Kraan, M.W.T. Werten, F.A. de Wolf, V. Subramaniam, B.J. Ravoo, D.N. Reinhoudt

    Research output: Contribution to journalArticleAcademicpeer-review

    102 Citations (Scopus)

    Abstract

    We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive islands, the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion
    Original languageEnglish
    Pages (from-to)6290-6297
    JournalChemistry-A European Journal
    Volume12
    Issue number24
    DOIs
    Publication statusPublished - 2006

    Keywords

    • self-assembled monolayers
    • mammalian-cells
    • soft lithography
    • surfaces
    • networks
    • gold
    • microelectrodes
    • environments
    • attachment
    • deposition

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  • Cite this

    Rozkiewicz, D. I., Kraan, Y., Werten, M. W. T., de Wolf, F. A., Subramaniam, V., Ravoo, B. J., & Reinhoudt, D. N. (2006). Covalent Microcontact Printing of Proteins for Cell Patterning. Chemistry-A European Journal, 12(24), 6290-6297. https://doi.org/10.1002/chem.200501554