Abstract
Glycans are crucial for the structure and function of anaerobic granular sludge in wastewater treatment. Yet, there is limited knowledge regarding the microorganisms and biosynthesis pathways responsible for glycan production. In this study, we analysed samples from anaerobic granular sludges treating papermill and brewery wastewater, examining glycans composition and using metagenome-assembled genomes (MAGs) to explore potential biochemical pathways associated with their production. Uronic acids were the predominant constituents of the glycans in extracellular polymeric substances (EPS) produced by the anaerobic granular sludges, comprising up to 60 % of the total polysaccharide content. MAGs affiliated with Anaerolineacae, Methanobacteriaceae and Methanosaetaceae represented the majority of the microbial community (30–50 % of total reads per MAG). Based on the analysis of MAGs, it appears that Anaerolinea sp. and members of the Methanobacteria class are involved in the production of exopolysaccharides within the analysed granular sludges. These findings shed light on the functional roles of microorganisms in glycan production in industrial anaerobic wastewater treatment systems.
Original language | English |
---|---|
Article number | 121240 |
Journal | Water Research |
Volume | 252 |
DOIs | |
Publication status | Published - 15 Mar 2024 |
Keywords
- Anaerobic granular sludge
- EPS biosynthesis pathways
- Extracellular polymeric substances
- Glycans
- Metagenomics
Fingerprint
Dive into the research topics of 'Coupling extracellular glycan composition with metagenomic data in papermill and brewery anaerobic granular sludges'. Together they form a unique fingerprint.Datasets
-
Microbiome and glycans of anaerobic granular sludge
Doloman, A. (Creator), de Bruin, S. (Creator), van Loosdrecht, M. C. M. (Creator), Machado de Sousa, D. (Creator) & Lin, Y. (Creator), Wageningen University & Research, 9 Nov 2023
https://www.ebi.ac.uk/ena/browser/view/PRJEB62884
Dataset