Correlation between protection against sepsis by probiotic therapy and stimulation of a novel bacterial phylotype

J. Gerritsen, H.M. Timmerman, S. Fuentes, L.P. van Minnen, H. Panneman, S.R. Konstantinov, F.M. Rombouts, H.G. Gooszen, L.M.A. Akkermans, H. Smidt, G.T. Rijkers

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14 Citations (Scopus)

Abstract

Prophylactic probiotic therapy has shown beneficial effects in an experimental rat model for acute pancreatitis on the health status of the animals. Mechanisms by which probiotic therapy interferes with severity of acute pancreatitis and associated sepsis, however, are poorly understood. The aims of this study were to identify the probiotic-induced changes in the gut microbiota and to correlate these changes to disease outcome. Duodenum and ileum samples were obtained from healthy and diseased rats subjected to pancreatitis for 7 days and prophylactically treated with either a multispecies probiotic mixture or a placebo. Intestinal microbiota was characterized by terminal-restriction fragment length polymorphism (T-RFLP) analyses of PCR-amplified 16S rRNA gene fragments. These analyses showed that during acute pancreatitis the host-specific ileal microbiota was replaced by an “acute pancreatitis-associated microbiota.” This replacement was not reversed by administration of the probiotic mixture. An increase, however, was observed in the relative abundance of a novel bacterial phylotype most closely related to Clostridium lituseburense and referred to as commensal rat ileum bacterium (CRIB). Specific primers targeting the CRIB 16S rRNA gene sequence were developed to detect this phylotype by quantitative PCR. An ileal abundance of CRIB 16S rRNA genes of more than 7.5% of the total bacterial 16S rRNA gene pool was correlated with reduced duodenal bacterial overgrowth, reduced bacterial translocation to remote organs, improved pancreas pathology, and reduced proinflammatory cytokine levels in plasma. Our current findings and future studies involving this uncharacterized bacterial phylotype will contribute to unraveling one of the potential mechanisms of probiotic therapy.
Original languageEnglish
Pages (from-to)7749-7756
JournalApplied and Environmental Microbiology
Volume77
Issue number21
DOIs
Publication statusPublished - 2011

Keywords

  • severe acute-pancreatitis
  • ribosomal-rna
  • necrotizing pancreatitis
  • clinical-course
  • sequence data
  • overgrowth
  • gut
  • translocation
  • microbiota
  • cirrhosis

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  • Datasets

    Genome sequence of the rat gut bacterium Romboutsia ilealis CRIB

    Gerritsen, J. (Creator), Timmerman, H. M. (Creator), Fuentes, S. (Creator), van Minnen, L. P. (Creator), Panneman, H. (Creator), Konstantinov, S. R. (Creator), Rombouts, F. M. (Creator), Gooszen, H. G. (Creator), Akkermans, L. M. A. (Creator), Smidt, H. (Creator) & Rijkers, G. T. (Creator), Wageningen University, 31 Mar 2017

    Dataset

    Cite this

    Gerritsen, J., Timmerman, H. M., Fuentes, S., van Minnen, L. P., Panneman, H., Konstantinov, S. R., Rombouts, F. M., Gooszen, H. G., Akkermans, L. M. A., Smidt, H., & Rijkers, G. T. (2011). Correlation between protection against sepsis by probiotic therapy and stimulation of a novel bacterial phylotype. Applied and Environmental Microbiology, 77(21), 7749-7756. https://doi.org/10.1128/AEM.05428-11