Convenient synthesis of lactuloselysine and its use for LC-MS analysis in milk-like model systems

Francesco Vinale, Simona Maria Monti, Barbara Panunzi, Vincenzo Fogliano*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The synthesis of the Amadori product lactuloselysine [N(ε)(1-deoxy-D- lactulosyl-1)-L-lysine] was obtained starting from FMOC-lysine-OH (N(α)-9- fluorenylmethoxy-carbonyl-N(ε)H2-L-lysine-OH) and lactose. Compound identity was confirmed by MALDI-ToF, electrospray, and NMR analysis. A selective LC-MS procedure which allowed the detection of lactuloselysine up to 10 ng mL-1 was set up and used to follow the formation of the compound in a lactose-lysine model system; quantification of this molecule after complete enzymatic hydrolysis of whey-proteins from milk samples was also performed.

Original languageEnglish
Pages (from-to)4700-4706
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume47
Issue number11
DOIs
Publication statusPublished - Nov 1999
Externally publishedYes

Keywords

  • Lactuloselysine
  • LC-MS
  • Maillard reaction
  • Milklike model systems

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