Control of Strobilurin Fungicides in Wheat Using Direct Analysis in Real Time Accurate Time-of-Flight and Desorption Electrospray Ionization Linear Ion Trap Mass Spectrometry

J. Schurek, L. Vaclavik, H. Hooijerink, O. Lacina, J. Poustka, M. Sharman, M. Caldow, M.W.F. Nielen, J. Hajslova

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103 Citations (Scopus)

Abstract

Ambient mass spectrometry has been used for the analysis of strobilurin residues in wheat. The use of this novel, challenging technique, employing a direct analysis in a real time (DART) ion-source coupled with a time-of-flight mass spectrometer (TOF MS) and a desorption electrospray ionization (DESI) source coupled with a linear ion trap tandem MS (LIT MSn), permitted a direct screen of the occurrence of target fungicides in treated grains in less than 1 min. For quantification purpose by DART-TOF MS, an ethyl acetate extract had to be prepared. With the use of a prochloraz as an internal standard, the performance characteristics obtained by repeated analyses of extract, spiked at 50 ¿g kg¿1 with six strobilurins (azoxystrobin, picoxystrobin, dimoxystrobin, kresoxim-methyl, pyraclostrobin, and trifloxystrobin), were in the following range: recoveries 78¿92%, repeatability (RSD) 8¿15%, linearity (R2) 0.9900¿0.9978. The analysis of wheat with incurred strobilurin residues demonstrated good trueness of data generated by the DART-TOF MS method; the results were in a good agreement with those obtained by the conventional approach, i.e., by the QuEChERS sample handling procedure followed by identification/quantification employing high-performance liquid chromatography coupled with tandem mass spectrometry (LC¿MS/MS). Tandem mass spectrometry using DESI-LIT MSn provided a sufficient number of product ions for confirmation of the identity of azoxystrobin and pyraclostrobin in incurred wheat samples.
Original languageEnglish
Pages (from-to)9567-9575
JournalAnalytical Chemistry
Volume80
Issue number24
DOIs
Publication statusPublished - 2008

Keywords

  • liquid-chromatography
  • pesticide-residues
  • ambient conditions
  • food
  • azoxystrobin
  • vegetables
  • matrices
  • fruits
  • methyl
  • dart

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