Abstract
Perception of pathogen-associated molecular patterns (PAMPs)
by patternrecognition receptors (PRRs) plays an important role
in plant innate immunity. In Arabidopsis, the leucine-rich
repeat receptor kinases (LRR-RKs) EFR and FLS2 mediate
recognition of the bacterial PAMPs EF-Tu and flagellin,
respectively. Only limited knowledge exists on how EFR and
FLS2 function at the molecular level. Here, we identify by
forward genetics the Arabidopsis stromalderived factor 2
(SDF2) as being required for responses mediated by EFR and
FLS2. Sdf2-2 mutants are more susceptible to bacteria fungi,
indicating that other, yet unknown PRRs also require SDF2.
SDF2 resides in the endoplasmic reticulum (ER) in a complex
with ERdj3B and the luminal binding protein (BiP). ERdj3B
and BiP are components of the ER-quality control (ER-QC),
and EFR accumulation is impaired in the sdf2 mutant. Erdj3b
mutants were independently identified in our screen, and loss
of ERdj3B function leads to compromised EFR and FLS2
responses. Additionally, sdf2 and erdj3b mutants were
impaired in the unfolded protein response induced by the drug
tunicamycin. Our results demonstrate that several
surface-exposed PRRs require the ER complex SDF2-ERdj3B-BiP for their function, and provide the first
demonstration of a physiological requirement for ER quality
control in trans-membrane receptor function in plants.
by patternrecognition receptors (PRRs) plays an important role
in plant innate immunity. In Arabidopsis, the leucine-rich
repeat receptor kinases (LRR-RKs) EFR and FLS2 mediate
recognition of the bacterial PAMPs EF-Tu and flagellin,
respectively. Only limited knowledge exists on how EFR and
FLS2 function at the molecular level. Here, we identify by
forward genetics the Arabidopsis stromalderived factor 2
(SDF2) as being required for responses mediated by EFR and
FLS2. Sdf2-2 mutants are more susceptible to bacteria fungi,
indicating that other, yet unknown PRRs also require SDF2.
SDF2 resides in the endoplasmic reticulum (ER) in a complex
with ERdj3B and the luminal binding protein (BiP). ERdj3B
and BiP are components of the ER-quality control (ER-QC),
and EFR accumulation is impaired in the sdf2 mutant. Erdj3b
mutants were independently identified in our screen, and loss
of ERdj3B function leads to compromised EFR and FLS2
responses. Additionally, sdf2 and erdj3b mutants were
impaired in the unfolded protein response induced by the drug
tunicamycin. Our results demonstrate that several
surface-exposed PRRs require the ER complex SDF2-ERdj3B-BiP for their function, and provide the first
demonstration of a physiological requirement for ER quality
control in trans-membrane receptor function in plants.
| Original language | English |
|---|---|
| Title of host publication | ISMPMI International Congress abstracts, Quebec City, Canada, 19-23 July 2009 |
| Publisher | IS MPMI |
| Publication status | Published - 2009 |
| Event | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada - Duration: 19 Jul 2009 → 23 Jul 2009 |
Conference
| Conference | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada |
|---|---|
| Period | 19/07/09 → 23/07/09 |