Control of anthocyanin and non-flavonoid compounds by anthocyanin-regulating MYB and bHLH transcription factors in Nicotiana benthamiana leaves

N.S. Outchkourov, C.A. Carollo, M.V. Gomez Roldan, C.H. de Vos, H.J. Bosch, R.D. Hall, M.J. Beekwilder

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Coloration of plant organs such as fruit, leaves and flowers through anthocyanin production is governed by a combination of MYB and bHLH type transcription factors (TFs). In this study we introduced Rosea1 (ROS1, a MYB type) and Delila (DEL, a bHLH type), into Nicotiana benthamiana leaves by agroinfiltration. ROS1 and DEL form a pair of well-characterized TFs from Snapdragon (Antirrhinum majus), which specifically induce anthocyanin accumulation when expressed in tomato fruit. In N. benthamiana, robust induction of a single anthocyanin, delphinidin-3-rutinoside (D3R) was observed after expression of both ROS1 and DEL. Surprisingly in addition to D3R, a range of additional metabolites were also strongly and specifically up-regulated upon expression of ROS1 and DEL. Except for the D3R, these induced compounds were not derived from the flavonoid pathway. Most notable among these are nornicotine conjugates with butanoyl, hexanoyl, and octanoyl hydrophobic moieties, and phenylpropanoid-polyamine conjugates such as caffeoyl putrescine. The defensive properties of the induced molecules were addressed in bioassays using the tobacco specialist lepidopteran insect Manduca sexta. Our study showed that the effect of ROS1 and DEL expression in N. benthamiana leaves extends beyond the flavonoid pathway. Apparently the same transcription factor may regulate different secondary metabolite pathways in different plant species.
LanguageEnglish
Article number519
Number of pages9
JournalFrontiers in Plant Science
Volume5
DOIs
Publication statusPublished - 2014

Fingerprint

delphinidin
Nicotiana benthamiana
rutin
anthocyanins
transcription factors
flavonoids
nornicotine
agroinfiltration
Antirrhinum majus
leaves
fruits
Manduca sexta
phenylpropanoids
plant organs
putrescine
polyamines
secondary metabolites
tobacco
bioassays
Lepidoptera

Keywords

  • r2r3-myb gene family
  • mass-spectrometry
  • tomato fruit
  • arabidopsis-thaliana
  • biosynthesis
  • tobacco
  • accumulation
  • metabolomics
  • plants
  • health

Cite this

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title = "Control of anthocyanin and non-flavonoid compounds by anthocyanin-regulating MYB and bHLH transcription factors in Nicotiana benthamiana leaves",
abstract = "Coloration of plant organs such as fruit, leaves and flowers through anthocyanin production is governed by a combination of MYB and bHLH type transcription factors (TFs). In this study we introduced Rosea1 (ROS1, a MYB type) and Delila (DEL, a bHLH type), into Nicotiana benthamiana leaves by agroinfiltration. ROS1 and DEL form a pair of well-characterized TFs from Snapdragon (Antirrhinum majus), which specifically induce anthocyanin accumulation when expressed in tomato fruit. In N. benthamiana, robust induction of a single anthocyanin, delphinidin-3-rutinoside (D3R) was observed after expression of both ROS1 and DEL. Surprisingly in addition to D3R, a range of additional metabolites were also strongly and specifically up-regulated upon expression of ROS1 and DEL. Except for the D3R, these induced compounds were not derived from the flavonoid pathway. Most notable among these are nornicotine conjugates with butanoyl, hexanoyl, and octanoyl hydrophobic moieties, and phenylpropanoid-polyamine conjugates such as caffeoyl putrescine. The defensive properties of the induced molecules were addressed in bioassays using the tobacco specialist lepidopteran insect Manduca sexta. Our study showed that the effect of ROS1 and DEL expression in N. benthamiana leaves extends beyond the flavonoid pathway. Apparently the same transcription factor may regulate different secondary metabolite pathways in different plant species.",
keywords = "r2r3-myb gene family, mass-spectrometry, tomato fruit, arabidopsis-thaliana, biosynthesis, tobacco, accumulation, metabolomics, plants, health",
author = "N.S. Outchkourov and C.A. Carollo and {Gomez Roldan}, M.V. and {de Vos}, C.H. and H.J. Bosch and R.D. Hall and M.J. Beekwilder",
year = "2014",
doi = "10.3389/fpls.2014.00519",
language = "English",
volume = "5",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers",

}

Control of anthocyanin and non-flavonoid compounds by anthocyanin-regulating MYB and bHLH transcription factors in Nicotiana benthamiana leaves. / Outchkourov, N.S.; Carollo, C.A.; Gomez Roldan, M.V.; de Vos, C.H.; Bosch, H.J.; Hall, R.D.; Beekwilder, M.J.

In: Frontiers in Plant Science, Vol. 5, 519, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Control of anthocyanin and non-flavonoid compounds by anthocyanin-regulating MYB and bHLH transcription factors in Nicotiana benthamiana leaves

AU - Outchkourov, N.S.

AU - Carollo, C.A.

AU - Gomez Roldan, M.V.

AU - de Vos, C.H.

AU - Bosch, H.J.

AU - Hall, R.D.

AU - Beekwilder, M.J.

PY - 2014

Y1 - 2014

N2 - Coloration of plant organs such as fruit, leaves and flowers through anthocyanin production is governed by a combination of MYB and bHLH type transcription factors (TFs). In this study we introduced Rosea1 (ROS1, a MYB type) and Delila (DEL, a bHLH type), into Nicotiana benthamiana leaves by agroinfiltration. ROS1 and DEL form a pair of well-characterized TFs from Snapdragon (Antirrhinum majus), which specifically induce anthocyanin accumulation when expressed in tomato fruit. In N. benthamiana, robust induction of a single anthocyanin, delphinidin-3-rutinoside (D3R) was observed after expression of both ROS1 and DEL. Surprisingly in addition to D3R, a range of additional metabolites were also strongly and specifically up-regulated upon expression of ROS1 and DEL. Except for the D3R, these induced compounds were not derived from the flavonoid pathway. Most notable among these are nornicotine conjugates with butanoyl, hexanoyl, and octanoyl hydrophobic moieties, and phenylpropanoid-polyamine conjugates such as caffeoyl putrescine. The defensive properties of the induced molecules were addressed in bioassays using the tobacco specialist lepidopteran insect Manduca sexta. Our study showed that the effect of ROS1 and DEL expression in N. benthamiana leaves extends beyond the flavonoid pathway. Apparently the same transcription factor may regulate different secondary metabolite pathways in different plant species.

AB - Coloration of plant organs such as fruit, leaves and flowers through anthocyanin production is governed by a combination of MYB and bHLH type transcription factors (TFs). In this study we introduced Rosea1 (ROS1, a MYB type) and Delila (DEL, a bHLH type), into Nicotiana benthamiana leaves by agroinfiltration. ROS1 and DEL form a pair of well-characterized TFs from Snapdragon (Antirrhinum majus), which specifically induce anthocyanin accumulation when expressed in tomato fruit. In N. benthamiana, robust induction of a single anthocyanin, delphinidin-3-rutinoside (D3R) was observed after expression of both ROS1 and DEL. Surprisingly in addition to D3R, a range of additional metabolites were also strongly and specifically up-regulated upon expression of ROS1 and DEL. Except for the D3R, these induced compounds were not derived from the flavonoid pathway. Most notable among these are nornicotine conjugates with butanoyl, hexanoyl, and octanoyl hydrophobic moieties, and phenylpropanoid-polyamine conjugates such as caffeoyl putrescine. The defensive properties of the induced molecules were addressed in bioassays using the tobacco specialist lepidopteran insect Manduca sexta. Our study showed that the effect of ROS1 and DEL expression in N. benthamiana leaves extends beyond the flavonoid pathway. Apparently the same transcription factor may regulate different secondary metabolite pathways in different plant species.

KW - r2r3-myb gene family

KW - mass-spectrometry

KW - tomato fruit

KW - arabidopsis-thaliana

KW - biosynthesis

KW - tobacco

KW - accumulation

KW - metabolomics

KW - plants

KW - health

U2 - 10.3389/fpls.2014.00519

DO - 10.3389/fpls.2014.00519

M3 - Article

VL - 5

JO - Frontiers in Plant Science

T2 - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 519

ER -